| Cyclic nucleotide-gated channel proteins (CNGC) play an important role in Ca2+ signal transduction. Genome-wide identification of CNGC gene family members in tetraploid alfalfa (Medicago sativa "Xinjiangdaye") and analysis of their regulatory patterns under drought stress were carried out using bioinformatics and molecular biology research methods. The results showed that the Xinjiangdaye alfalfa genome contained 67 MsCNGC genes, unevenly distributed on 30 chromosomes, belonging to five subfamilies and containing 20 motifs, with a variety of action elements in their promoter regions in response to adversity stress. A total of 25 MsCNGC genes were expressed in the leaves of Xinjiangdaye alfalfa, and 23 of them were unevenly up-regulated under drought stress, among which MsCNGC59 was the highest expressed and the most multiplicatively up-regulated under drought stress.The proteins of MsCNGCs contained 29-120 phosphorylation sites, which could be phosphorylated by the LRR protein kinase, and the expression of MsLRRs and the genes of MsCNGCs showed a significant positive correlation. In addition, the expression of Ca2+ signal transduction-related genes MsCaM, MsCML and MsCDPK was found to be significantly up-regulated under drought stress. In summary, the response of tetraploid alfalfa MsCNGCs to drought stress includes transcriptional regulation and protein phosphorylation regulation, which can improve the drought resistance of alfalfa under drought stress by participating in the Ca2+ signal transduction pathway. This study provides a reference for further research on the molecular functions of the CNGC gene family in response to drought stress in Xinjiangdaye alfalfa. |