| Genome-wide identification of the NAC gene family in Apocynum pictum and its expression analysis under drought stress |
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| 投稿时间：2024-04-24 修订日期：2024-07-07 |
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| Key Words: Apocynum pictum, NAC gene family, drought stress, gene different expression |
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| Abstract: |
| Apocynum pictum Schrenk, a semi-shrub of the Apocynaceae family with a wide distribution throughout the Tarim Basin, which has significant ecological, medicinal, and economic values. The NAC gene family is one of the plant-specific gene families, widely involved in regulating plant growth and development as well as plant responses to abiotic stress. Based on the genome data of Apocynum pictum, this study identified 53 members of the NAC gene family in A. pictum, and conducted predictive analyses on their chromosomal distribution, phylogenetic relationships, gene and protein structures, physicochemical properties and functions, as well as cis-acting elements, combined with transcriptome data analysis of NAC gene expression under drought stress. The results revealed that the ApNAC gene family of Apocynum pictum is distributed on 10 chromosomes, including 7 pairs of collinearity genes formed from segmental duplications and 2 pairs of tandem duplicated genes. The 53 members of the ApNAC family can be classified into 15 subfamilies, each member containing more than 5 conserved motifs, with similar gene structures within the same subfamily. The NAC proteins of Apocynum pictum are hydrophilic protein, with subcellular localization results indicating their functional role as transcription factors, enriched in DNA-specific binding functions as well as regulating development in xylem, leaves, and cell walls. Analysis of cis-acting elements in the promoter region revealed the association of the ApNAC gene family with regulation of abscisic acid, drought stress, and light responses. Under drought stress induced by 30% PEG-6000, 26 members of ApNAC showed differential expression at different stages, with 21 members upregulated and 5 members downregulated. This study provides information on the evolution and potential functions of ApNAC genes, laying the foundation for further research on the drought resistance mechanism of the NAC family. |
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