In order to explore the drought tolerance of sorghum germplasm at germination stage and screen out sorghum germplasm with strong drought tolerance, 30 sorghum germplasms were treated with different concentrations of simulated drought stress using PEG-6000 as penetrant in this study. The results showed that 15 % PEG-6000 had the best stress effect on sorghum germplasm. Under the stress of this treatment concentration, the drought tolerance of 279 sorghum germplasm at germination stage was comprehensively evaluated by various analysis methods. The results showed that the coefficient of variation of 9 drought tolerance indexes ranged from 21.61 % to 52.62 %, and the germination potential, germination rate and germination index were determined according to the correlation degree of each index. Through principal component analysis, the nine drought tolerance indexes were integrated into three independent comprehensive indexes ( principal component values ), and the cumulative contribution rate of these three principal component values was 88.044 %. Based on principal component analysis, the comprehensive evaluation D value of drought tolerance of sorghum germplasm at germination stage was calculated by membership function method. At the same time, based on grey correlation analysis, the relative sticking progress of drought tolerance of sorghum germplasm at germination stage was calculated by DTOPSIS method, and then the D value and relative sticking progress were averaged to obtain a new comprehensive evaluation value. According to the new comprehensive evaluation value, 279 sorghum germplasms were divided into five categories by Euclidean distance and class average method, including 29 extremely drought-tolerant germplasms, 170 drought-tolerant germplasms, 52 moderate drought-tolerant germplasms, 12 drought-sensitive germplasms and 16 drought-sensitive germplasms. Through the screening of these sorghum germplasm, it is expected to provide a reference for the rapid identification and evaluation of drought tolerance during germination of sorghum germplasm. |