| To explore the role of VrWRKY50 gene in mung bean response to saline-alkali stress, analyze its protein structure and expression pattern under salt-alkali stress and prohexadione-calcium spray, In this study, 17 WRKY50 homologous proteins were analyzed by bioinformatics. qRT-PCR was used to determine the expression pattern of VrWRKY50 gene in mung bean under saline-alkali stress and prohexadione-calcium spray. The results showed that all 17 WRKY50 proteins were hydrophilic proteins, and Motif 1 was the main conserved motif of WRKY50. WRKY50 gene contained 3-4 exons, and the promoter region contained 9 cis-acting elements related to growth and development, stress response, hormone response, etc. The protein homology of mung bean VrWRKY50 and adzuki bean VaWRKY50 was the highest. Salt and alkali stress significantly up-regulated VrWRKY50 gene expression in mung bean, increasing 13.91-fold (6th day) and 7.15-fold (8th day) in leaves and roots, respectively. The peak time of VrWRKY50 gene expression was advanced to 1 day by spraying prohexadione-calcium. The expression levels in leaves and roots were 47.70 times and 3.52 times of those in saline-alkali control, respectively. |