| 何道文,孙辉,黄雪菊.利用N-15自然丰度法研究固氮植物生物固氮量[J].干旱地区农业研究,2004,(1):132~137 |
| 利用N-15自然丰度法研究固氮植物生物固氮量 |
| A Review on quantifying biological nitrogen fixation based on N-15 natural abundance method |
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| DOI：10.7606/j.issn.1000-7601.2004.01.30 |
| 中文关键词: 农林复合经营 生物固氮 N-15自然丰度法 氮素循环 |
| 英文关键词:agroforestry biological nitrogen fixation N-15 natural abundance nitrogen cycling |
| 基金项目:国际山地中心(ICIMOD)ATSCFS项目(1991-2001) |
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| 摘要点击次数: 54 |
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| 中文摘要: |
| 生物固氮对全球生态系统氮素循环和全球变化有着重要影响,但是由于测定方法的原因,过去对生物固氮,特别是多年生固氮植物的共生固氮的定量研究不足。在对目前国际上日益受到重视的用N-15自然丰度法研究固氮植物生物固氮量的研究进展进行初步总结的基础上,探讨了影响该方法准确性的一些主要因素。N-15自然丰度法克服了传统的差值法、N-15同位素稀释法和乙烯还原法等的不足,可野外原位定量研究生物固氮,如果对各影响因子加以系统考虑,合理解决参比植物选择和固氮植物取样部位等方面的问题,该方法可较为准确地原位测定人工生态系统中固氮作物和固氮树种的生物固氮量。但是在天然生态系统中由于植物有效氮源的多样性以及其它影响因素较复杂,应用难度较大。 |
| 英文摘要: |
| Biological nitrogen fixation (BNF) is strongly associated with global nitrogen cycling and global changes due to its effects on release of greenhouse gas. However, quantifying BNF is very difficult because of the inherent defect of the study methods such as N-15 dilution method and ethylene reduction method. N-15 natural abundance method is adopted more and more widespreddly in quantitative study of BNF, which exploits naturally occurring differences of N-15 between the available N in the soil and the N2 in the atmosphere. The principles and technical details of this method are reviewed in the paper, and the contributing factors of its accuracy, such as determination of B value, δ15N in reference plants and sampling tissues of nitrogen-fixation plants, are also discussed. The paper suggests that N-15 natural abundance may be a promising method for quantification of BNF in man-made ecosystems, such as agriculture and agroforesrty system, by designation of reasonable sampling strategies. |
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