| 孙耀中,东方阳,郭学民,刘永军,杨晓玲,吉志新.干旱胁迫下转甜菜碱醛脱氢酶基因水稻花后生理特性及产量构成[J].干旱地区农业研究,2005,(5):108~113 |
| 干旱胁迫下转甜菜碱醛脱氢酶基因水稻花后生理特性及产量构成 |
| Physiological characteristics and yield components in betaine aldehyde dehydrogenase transgenic rice after flowering under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2005.05.21 |
| 中文关键词: 干旱胁迫 转基因水稻 丙二醛含量 SOD活性 脯氨酸含量 失水率 产量构成 |
| 英文关键词:drought stress transgenic rice MDA content SOD activity proline content water-losing rate yield components |
| 基金项目:河北省秦皇岛市科学技术局资助项目 |
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| 中文摘要: |
| 在干旱胁迫条件下,对转甜菜碱醛脱氢酶(BADH)基因水稻品系51-22、51-15,受体亲本中花8号(ck1),旱297(ck2)等4个品种(系)开花后剑叶的丙二醛含量、SOD酶活性、脯氨酸含量和24h失水率进行了测定分析。结果表明:在开花后10~25d,两个转BADH基因水稻品系的MDA含量均低于中花8号和旱297;开花后5~15d,4个品种(系)的SOD酶活性呈现逐渐上升趋势,而后迅速下降。在开花后10~25d,两个转BADH基因水稻品系的SOD酶活性显著高于中花8号;两个转BADH基因水稻品系的脯氨酸含量的变化虽不稳定,但显著高于中花8号;两个转BADH基因品系24h失水率均低于中花8号。相关分析表明:MDA含量、SOD酶活性和脯氨酸(Pro)含量存在一定程度的线性负相关,SOD酶活性与脯氨酸(Pro)含量存在显著线性正相关;MDA含量与稻谷产量呈显著线性负相关;SOD酶活性、脯氨酸(Pro)含量与稻谷产量呈线性正相关,但未达显著。 |
| 英文摘要: |
| Under drought stress, the MDA content,SOD activity,proline content and 24 h water losing rate in the flag leaves of Betaine Aldehyde Dehydrogenase (BADH) transgenic rice strains 51-15, 51-22 and their parents (Zhonghua8, Han297) were measured after flowering. The results indicated that both 51-15 and 51-22 had lower MDA content than Zhonghua8 and Han297 during 10~25 d after flowering; The SOD activity of the four strains went up gradually and then went down suddenly during 5~15 d after flowering; The SOD activity of both 51-15 and 51-22 is significantly higher than that of Zhonghua8 during 10~25 d after flowering; Though it changed unstably, the proline content of both 51-15 and 51-22 was remarkably higher than that of Zhonghua8; The 24 h water-losing rate of both 51-15 and 51-22 was lower than that of Zhonghua8. The results of correlation analysis showed that MDA content was negatively related to SOD activity and proline content; There was a significant linear positive correlation between SOD activity and proline content; There was a significant linear negative correlation between MDA content and rice yield; SOD activity and proline content were positively related to rice yield. |
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