| 邓光存,彭励,杨彩荣,吴晓玲.不同激素对银柴胡细胞生长及营养成分消耗的影响[J].干旱地区农业研究,2006,(6):107~111 |
| 不同激素对银柴胡细胞生长及营养成分消耗的影响 |
| The effect of the different hormones on the growth and the consuming of nutrition elements of the starwort cell |
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| DOI：10.7606/j.issn.1000-7601.2006.06.24 |
| 中文关键词: 银柴胡 细胞悬浮培养 动力学 |
| 英文关键词:starwort cell suspension culture metabolizing kinetics |
| 基金项目:宁夏教育厅资助项目(JY2002110)； 宁夏大学自然基金资助项目(NS0404) |
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| 中文摘要: |
| 采用液体培养基悬浮培养的方法,研究4种不同激素培养基对银柴胡悬浮细胞的生长特性及营养物质的代谢规律的影响,结果表明:当培养到16 d时,M4处理(MS+6-BA 0.5 mg/L+2,4-D0.5 mg/L+NAA 0.5mg/L)的细胞鲜重、干重、细胞数目均明显高于其他处理;而M4处理的可溶性糖的含量在16 d时处于最低水平;M4处理的电导率在整个培养过程中均高于其他处理;培养到4 d时各处理的磷已消耗60%以上,表明磷是银柴胡细胞生长的限制性因子;硝态氮的吸收主要集中在4 d以后。 |
| 英文摘要: |
| The effects of different hormone treatments on the growth and metabolizing of the starwort cell nutrition were studied.The results showed that the numbers, the dry weight and the fresh weight of starwort cells treated with M4 (MS+6-BA 0.5 mg/L+2, 4-D 0.5 mg/L+NAA 0.5 mg/L) medium were the highest on the 16 th day, while the content of sugar was the lowest.Moreover, electronic leakage of M4 treatment was the highest during the culture period.The content of phosphor which used up to 60% from 0～4 day in mediums was the key factor of starwort cell growth, while starwort cell absorbed nitrate nitrogen mostly after 4 days. |
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