| 李小玲,刘雅莉,王跃进,华智锐,陈远.亚洲百合遗传转化受体系统的建立[J].干旱地区农业研究,2007,(1):219~224 |
| 亚洲百合遗传转化受体系统的建立 |
| Establishment of the acceptor system for the gene transformation of Lilium Asiatic hybrids |
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| DOI：10.7606/j.issn.1000-7601.2007.01.46 |
| 中文关键词: 亚洲百合 遗传转化 受体系统 |
| 英文关键词:Lilium Asiatic gene transformation acceptor system |
| 基金项目:国家自然科学基金项目(30371013) |
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| 摘要点击次数: 474 |
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| 中文摘要: |
| 以亚洲百合“精粹”(Lilium Asiatic‘Elite’)的鳞片、再生形成的叶片和鳞片叶为外植体,进行了不定芽的诱导分化、试管苗小鳞茎诱导、卡那霉素抗性筛选等研究,建立了亚洲百合遗传转化受体系统。结果表明:鳞片诱导最佳分化培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,试管苗小鳞茎诱导培养基为MS+IBA 0.5mg/L+70 g/L蔗糖,试管苗叶片分化培养基为MS+TDZ 1.0 mg/L+NAA 0.2 mg/L,试管苗鳞片叶分化培养基为MS+6-BA 2.0 mg/L+2,4-D 0.1 mg/L。抗生素敏感性试验表明,百合叶片的卡那霉素选择压为80 mg/L,鳞片叶为115 mg/L。 |
| 英文摘要: |
| Using scales and petioles of Lilium Asiatic hybrid as the study subject,the acceptor system for the gene transformation of Lilium Asiatic hybrids was established.The results showed that the medium suitable for inducing the scales to differentiate Adventitious buds was MS+6-BA 2.0 mg/L+NAA 0.2(mg/L,) the medium suitable for small bulb induction was MS+IBA 0.5 mg/L+Sucrose 70 g/L, the medium suitable for the leaves of the test-tube plantlets to differentiate adventitious buds was MS+TDZ 1.0 mg/L+NAA 0.2(mg/L,) and the medium suitable for the scale leaves of the test-tube plantlets to differentiate adventitious buds was MS+6-BA 2.0 mg/L+2,4-D 0.1 mg/L.The concentrations of the antibiotics for different parts of the test-tube plantlets in their cultures were determined,the concentration of Kanamycin for the petioles was 80 mg/L and for the scales 115 mg/L. |
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