杜喜梅,张俊莲,王蒂,黄鹏,崔炎森,王丽,张天宇.烟草试管苗对盐胁迫的生理响应[J].干旱地区农业研究,2007,(4):238~242
烟草试管苗对盐胁迫的生理响应
The physiological responses of tobacco plantlets in vitro by salt stress
  
DOI:10.7606/j.issn.1000-7601.2007.04.47
中文关键词:  烟草试管苗  盐胁迫  生理响应
英文关键词:tobacco plantlets in vitro  salt stress  physiological effect
基金项目:甘肃省科技攻关课题(2GS054-A41-005-01)
作者单位
杜喜梅 甘肃农业大学农学院, 甘肃 兰州 730070 
张俊莲 甘肃农业大学农学院, 甘肃 兰州 730070甘肃省作物遗传改良与种质创新重点实验室 甘肃 兰州 730070 
王蒂 甘肃农业大学农学院, 甘肃 兰州 730070甘肃省作物遗传改良与种质创新重点实验室 甘肃 兰州 730070 
黄鹏 甘肃农业大学农学院, 甘肃 兰州 730070 
崔炎森 甘肃农业大学农学院, 甘肃 兰州 730070 
王丽 甘肃农业大学农学院, 甘肃 兰州 730070 
张天宇 甘肃农业大学农学院, 甘肃 兰州 730070 
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中文摘要:
      利用不同浓度的NaCl对烟草试管苗进行了20 d胁迫,结果发现:随着盐浓度的增加,烟草试管苗的生长明显受阻;根、茎、叶中K+的含量呈极显著的下降趋势,Na+的含量大幅度增加;叶片中叶绿素的含量下降,但丙二醛的含量增加,质膜透性增大,脯氨酸大量积累,且脯氨酸含量与丙二醛含量和质膜透性间呈极显著的正相关,表明盐胁迫下脯氨酸积累的多少可反映烟草试管苗的伤害程度。本研究认为离子伤害、膜透性增大和叶绿素含量下降是影响盐胁迫下烟草试管苗生长的原因。
英文摘要:
      The tobacco plantlets in Vitro were treated with different concentrations of NaCl in 20 days. The result indicated that the plant growth was retarded steadily with the increase of salt concentration; In this case, the content of K+ reduced and the content of Na+ increased in root, stem and leaf; In leaf, content of chlorophyll reduced, but the content of malonaldehyde(MDA), membrane permeability and proline increased, moreover the content of proline showed significant positive correlation with the content of malonaldehyde(MDA) and membrane permeability. It showed that the accumulative content of praline could reflect the tobacco plantlets' hurt degree. The study indicated that either the hurt of ion, the increase of membrane permeability or the reduction of chlorophyll affected the growth of tobacco plantlets in vitro of salt treatment.
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