| 张敏娟,刘萌娟,赵惠贤.陕西省农家种大豆资源11S 和7S 球蛋白含量分析[J].干旱地区农业研究,2008,(1):187~193 |
| 陕西省农家种大豆资源11S 和7S 球蛋白含量分析 |
| The analysis of 7S and11S globulin content of soybean landraces in Shaanxi |
| |
| DOI：10.7606/j.issn.1000-7601.2008.01.34 |
| 中文关键词: 大豆 农家种 球蛋白 SDS-PAGE |
| 英文关键词:Glycing max(L.) landrace globulin SDS-PAGE |
| 基金项目:农业部种质资源保护项目(NB04-22-6)； 西北农林科技大学青年学术骨干支持计划 |
|
| 摘要点击次数: 208 |
| 全文下载次数: 150 |
| 中文摘要: |
| 利用SDS-PAGE分析了陕西省75份农家种大豆核心种质的11S和7S球蛋白亚基组成、相对百分含量和11S/7S比值,并对陕北、关中和陕南三个地区的资源材料进行了分析和比较。结果表明:大豆贮藏蛋白质由许多亚基组成,其中含量较高的有α′、α、β、A3、Acid和Basic;不同品种间大豆球蛋白组成几乎没有差异,但是各亚基的含量存在较大差异;75份供试材料的7S、11S球蛋白含量的平均值分别为36%和64%,变幅分别为28%48%和52%72%;11S/7S比值的变异幅度在1.092.53,平均值为1.78,高于2.0的种质有19个;陕北、关中、陕南三个地区农家种大豆群体11S/7S比值存在显著差异。 |
| 英文摘要: |
| The relative percentage content of 11S and 7S and the 11S/7S value of 75 soybean landraces from Shaanxi province were investigated by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) in this study.The results showed that,soybean storage protein have various subunits,in which α′,α, β,A3,Acid and Basic have a high content;Although the band type of soybean storage protein are basically the same,the content among different subunits of each landrace is significant difference.11S and 7S globulin average content were 36% and 64%,respectively,their variational range were from 28% to 48% and from 52% to 72%,respectively.11S/7S value ranged from 1.09 to 2.53,with an average of 1.78;11S/7S value was significantly different among the three cultivated regions. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| | |