黄诚梅,杨丽涛,江文,毕黎明,李杨瑞.聚乙二醇胁迫对甘蔗生长前期叶片水势及脯氨酸代谢的影响[J].干旱地区农业研究,2008,(1):205~208
聚乙二醇胁迫对甘蔗生长前期叶片水势及脯氨酸代谢的影响
Effects of water stress on water potential and proline metabolism in leaves of sugarcane （Saccharum officinarum L．） at early growth stage
  
DOI：10.7606/j.issn.1000-7601.2008.01.37
中文关键词:  甘蔗  PEG胁迫  水势  脯氨酸代谢
英文关键词:Saccharum officinarum L.  polyethylene glycol(PEG) stress  water potential  proline metabolism
基金项目:广西区科技厅科技创新能力与条件建设项目(桂科能0630006-5F)； 广西农业科学院科技发展基金(2004001)
作者单位
黄诚梅 广西农业科学院广西作物遗传改良生物技术重点开放实验室,广西 南宁 530007；广西大学农学院,广西 南宁 530005 
杨丽涛 广西大学农学院,广西 南宁 530005 
江文 广西大学农学院,广西 南宁 530005；广西农业科学院生物技术研究所,广西 南宁 530007 
毕黎明 广西大学农学院,广西 南宁 530005 
李杨瑞 广西农业科学院广西作物遗传改良生物技术重点开放实验室,广西 南宁 530007；广西大学农学院,广西 南宁 530005 
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中文摘要:
      ：以3个甘蔗品种‘桂糖119’(‘GT119’)、‘新台糖16号’(‘ROC16’)、‘新台糖22号’(‘ROC22’)为材料,利用PEG6000淋灌于甘蔗幼苗根部模拟土壤水分胁迫,研究其对植株叶片水势、脯氨酸含量、P5CS、δ-OAT、ProDH酶活性等的影响。结果表明,3个品种经胁迫处理,其叶片水势都较对照有所下降;对于游离脯氨酸含量的变化,‘ROC16’在处理后第1天含量就明显上升,‘GT119’在处理2 d后也开始上升,而‘ROC22’在处理后12 d内其含量仍增加不明显;吡咯啉-5-羧酸合成酶活性经胁迫处理都较未处理表现活跃,‘GT119’与‘ROC16’在胁迫处理后酶活性表现上升趋势,而‘ROC22’变化不明显;鸟氨酸转氨酶活性变化不明显;脯氨酸脱氢酶活性,‘GT119’与‘ROC16’在处理1 d后稍有上升但在7天后都有下降,而‘ROC22’在第7天酶活性就开始提高。
英文摘要:
      Three sugarcane(Saccharum officinarum L.) varieties,‘Guitang119’,‘ROC16’ and ‘ROC22’,were used as experimental materials.The plants were treated with polyethylene glycol(PEG) 6000 at the early growth stage.Effects of water stress on water potential,proline content,activities of Δ1-pyrroline-5-carboxylate synthetase(P5CS),ornithine-δ-aminotransferase(δ-OAT) and proline dehydrogenase(ProDH) of leaves were studied.The results showed that,under the stress treatment,leaf water potential of three varieties decreased.And free proline content of ‘ROC16’ increased 1 d after the starting of treatment,‘GT119’ also increased 2 d after the starting of treatment,while there was no difference between treatment and control for the variety ‘ROC22’ during 12 d.The same results for the activity of P5CS were obtained after the stress treatment,the activity of ‘GT119’ and ‘ROC16’ increased after treatment,but ‘ROC22’ was contrary.For the activities of δ-OAT,there was no remarkable difference between the treatment and control.The proline dehydrogenase(ProDH) activity of ‘GT119’ and ‘ROC16’ increased at first then decreased after 7 d,‘ROC22’ increased 7 d after the starting of treatment.
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