| 康国章,岳彩凤,官春云,韩巧霞,郭天财,朱云集,王永华.小麦淀粉合酶基因Ⅱ克隆及反义和RNAi载体构建[J].干旱地区农业研究,2008,(3):109~114 |
| 小麦淀粉合酶基因Ⅱ克隆及反义和RNAi载体构建 |
| Molecular cloning of starch synthase Ⅱ partial sequences and construction of its antisense and RNAi expression vectors |
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| DOI：10.7606/j.issn.1000-7601.2008.03.22 |
| 中文关键词: 普通小麦 淀粉合酶基因Ⅱ 表达载体 |
| 英文关键词:wheat （Triticum aestivum） starch synthase Ⅱ gene expression vectors |
| 基金项目:中国博士后科学基金(第三十九批)； 河南省教育厅自然科学基金(2006210007) |
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| 中文摘要: |
| 采用RT-PCR方法从小麦品种豫教2号的籽粒中克隆出淀粉合酶Ⅱ基因(Starch synthaseⅡ,SSⅡ)部分cDNA片段(600bp)(GenBank No.EF221761),同源性比较结果显示,它与GenBank上已报道的SSⅡ基因有高度同源性。以pCMBIA1301质粒为基础,构建了由35S启动子调控的SSⅡ基因的反义表达载体pCMBIA1301SSIIA;另外,还以pFGC5941质粒为基础,构建了SSⅡ基因的RNAi载体pFGC5941SSIIsa,这些载体的构建为研究此基因的功能奠定了基础。 |
| 英文摘要: |
| Starch synthase Ⅱ （SSⅡ） partial cDNA sequences （600bp） （GenBank No．EF221761） from grains of common wheat （ T riticum aestiv um Yujiao 2 cultuvar） was amplified by RT-PCR．The result demonstrated that the cloned SSⅡ gene sequences were99％ identified with the reported SSⅡ genes in Gen-Bank previously．In addition its antisense expression vector was constructed with pCMBIA1301 and RNAi vector was also constructed with pFGC5941．These constructed vectors will provide a good background to study the function of SSⅡ on biosynthesis of starch in wheat plants． |
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