| 龚宁,薛长雷,李玉平,杨公明.低浓度Cu和Cd复合污染对小白菜生理生化特性的影响[J].干旱地区农业研究,2008,(4):141~147 |
| 低浓度Cu和Cd复合污染对小白菜生理生化特性的影响 |
| Influence of combi ned pollution of Cu and Cd on the physiological and bioche mical characteristics of Brassica campestris ssp．chi nensis |
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| DOI：10.7606/j.issn.1000-7601.2008.04.27 |
| 中文关键词: 铜 镉 生理生化特性 小白菜 复合污染 |
| 英文关键词:Cu Cd combined pollution physiological and biochemical characteristics Brassicaca mpestris ssp．chinensis |
| 基金项目:广东省食品工业公共试验室开放课题支持(2006231) |
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| 中文摘要: |
| 采用盆栽试验,研究了不同质量浓度Cu(0,25,50,100,200 mg/kg)和Cd(0,1.0,10.0 mg/kg)及其复合污染对小白菜生理生化指标的影响。以不添加Cu和Cd为对照组。结果表明,小白菜外观正常,没有出现Cd中毒症状,但是Cd-Cu复合处理组小白菜的生物量、叶绿素a、叶绿素总量、叶绿素a/b值都低于对照及单一处理组,Cu、Cd复合处理对叶绿素a的影响程度大于叶绿素b;复合处理组的细胞膜透性明显低于单一处理组,硝酸还原酶(NR)活性显著高于对照及单一处理组,过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性高于相同浓度Cd单一处理组。 |
| 英文摘要: |
| By pot experi ment the paper studied the effects of heavy metal pollution （Cu Cd and Cu ＋Cd） on the physiological and biochemical characteristics of plant （Brassica campestris ssp．chinensis）.The test soil contains one of five levels of Cu（0 25 50 100or 200mg／kg） and one of three levels of Cd（0 1 10mg／kg）. Harvest and analysis of leaf was after 40days.During the 40—day experi ment Brassica campestris ssp．chinensis survivedin the soil spiked up to 10mg／kg Cd and 200mg／kg Cu showing no visual heavy metal toxictiy sy mptoms such as necrosis and whitish-brown chlorosis.The biomass is negatively affected by the mixture of Cu and Cd. In the absence of Cd the relative plasmolemma per meability was sti mulated withincreasing Cu concentration but it was first inhibited and then sti mulated withincreasing concentration of Cu by the presence of Cd. Cu or Cd strongly reduced theleaf contents of chlorophyll （Chl） a and the ratio of Chl a and Chl b.The NRactivities did not vary withincreasing Cu concentration at Cd ＝0mg／kg.However the activities were first sti mulated and theninhibited withincreasing Cu concentration at Cd ＝1or 10mg／kg.The NRactivities were particularly increased at the concentration of Cu ＝200mg／kg.The sensibility of antioxidant enzy mes to increasing Cd and Cu concentration are different to enzy mes kinds.The SOD activity was first sti mulated and then inhibited with increasing concentration of Cu without Cd and that was sti mulated with increasing concentration of Cu at Cd ＝1or 10mg／kg.There were si milar responses in PODand SODactivities and the PODactivity was sti mulated with increasing concentration of Cd under same concentration of Cu.Increasing Cu concentration in soil significantly increased the activity of CAT and at the same Cu concentration the CAT activity was inhibited with increasing concentration of Cd. |
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