| 盛海彦,李松龄,杨改河,冯永忠,曹广民.高山草甸土壤放线菌分离方法研究[J].干旱地区农业研究,2008,(6):101~105 |
| 高山草甸土壤放线菌分离方法研究 |
| Study on isolation methods of actinomycetes from Alpine Meadow soils |
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| DOI：10.7606/j.issn.1000-7601.2008.06.19 |
| 中文关键词: 高山草甸土 放线菌 分离方法 样品预处理 |
| 英文关键词:alpine meadow soil actinomycete isolation method pre-treatment |
| 基金项目:中国生态网络台站项目； 青海省重大科技攻关项目(2002-N-106) |
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| 中文摘要: |
| 采用平板涂抹分离法研究了不同分离培养基及样品预处理对高山草甸土放线菌分离的效果。结果表明:土样在120℃干热处理1 h后其悬浮液加0.05%SDS(十二烷基磺酸钠)和6%酵母膏,40℃振荡30 min,能促进放线菌孢子萌发,增加放线菌的分离数量,并抑制杂菌生长,是高山草甸土放线菌分离时理想的预处理方法。改良HV琼脂和高氏1号加重铬酸钾(50 mg/L)培养基是较好的分离培养基。 |
| 英文摘要: |
| The isolation effect of different basic culture media and pre-treatments on actinomycetes from alpine meadow was studied by using plate paint isolation methods.The results showed that: the best pre-treatments methods of the soil samples on actinomycete isolation is to treat with 120℃ for 1 hour,then to add 6% yeast extract and 0.05% SDS and vibrate at 40℃ for 30 minutes.It could promote actinomycetes spore sprouting to increase the quantity of actinomycetes and inhibit the development of other microorganisms.Meanwhile,the gause,s starch agar media added with K2Cr2O7(50 mg/L) and modified HV media were best culture medium for isolation of actinomycetes. |
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