顾庆龙,莫亿伟,涂庆华,李运合,胡玉林,习金根.盐胁迫下NO对玉米幼苗生理特性的影响[J].干旱地区农业研究,2009,27(1):157~162
盐胁迫下NO对玉米幼苗生理特性的影响
Enhancement of exogenous nitric oxide to maize seedlings’resistance to salt stress
  
DOI：10.7606/j.issn.1000-7601.2009.01.31
中文关键词:  玉米幼苗  一氧化氮  盐胁迫  硝普钠  活性氧
英文关键词:maize seedling  nitric oxide  salt stress  sodium nitroprusside  reactive oxygen
基金项目:中央级公益性科研院所基本科研业务专项(2008-05)
作者单位
顾庆龙 Yangzhou College of Education, Yangzhou, Jiangsu 225009, China 
莫亿伟 South Subtropical Crops Research Institute Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091,China 
涂庆华 Fuzhou Vocational and Technical College, Fuzhou, Jiangxi 344000, China 
李运合 South Subtropical Crops Research Institute Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091,China 
胡玉林 South Subtropical Crops Research Institute Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091,China 
习金根 South Subtropical Crops Research Institute Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091,China 
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中文摘要:
      为探讨外源一氧化氮(NO)供体和清除剂对玉米幼苗抗盐性的影响,用50 mmol/L的NaCl浇灌4叶期的玉米幼苗造成盐害,同时加入10~100μmol/L的NO生成剂硝普钠(SNP)或清除剂CPTIO。试验结果表明,加入NO供体SNP可以提高玉米叶片中NO的含量,降低超氧阴离子的产生速率,降低叶片脂氧合酶活性(LOX)、丙二醛(MDA)含量和相对电导率,提高叶片相对含水量、延缓叶绿素的降解和提高植株干重;在SNP不同的使用浓度中,以40μmol/L的SNP效果最好,当SNP浓度过低和过高时均达不到理想的效果。当NO被CPTIO清除后则加速玉米的受害程度。因此适当浓度NO能够减轻玉米受盐胁迫的伤害。
英文摘要:
      To investigate whether maize (Zea mays L.)seedlings’ tolerance to salt stress could be enhanced by exogenous nitric oxide,stress was made to maize seedlings at 4-leaf stage with 50 mmol/L of NaCl,and meanwhile various content of NO donor sodium nitroprusside(SNP) and NO scavenger CPTIO(2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt) were added.The results showed that,with its concentration at 10～100 μmol/L SNP,the NO donor,could increase significantly the content of nitric oxide(NO),chlorophyll and relative water content in leaves,but decreased the super oxidtase redicals generating rate and LOX activities of leaves,meanwhile the content of malondiadehyde(MDA) and relative conductance were reduced but the dry weight of maize seedling was increased,and 40 μmol/L SNP had the most satisfactory effectt,while higher or lower SNP content had no such satisfactory effect.The stress to the growth of maize was accelerated when NO was eliminated by NO scavenger(CPTIO). So it could be concluded that proper content of NO could enhance maize seedlings’ resistance to salt stress.While the effect was distinctly different when treated with 10～100 μmol/L NO scavenger CPTIO.These results suggested that the propriety NO content can enhance maize seedlings’ resistance to salt stress.
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