| 马钰,蒋斌,王庆俐,张林生.反相高效液相色谱法测定PEG 胁迫下小麦幼苗中甜菜碱的含量变化[J].干旱地区农业研究,2009,27(1):190~194 |
| 反相高效液相色谱法测定PEG 胁迫下小麦幼苗中甜菜碱的含量变化 |
| The determination of Betaines in wheat seedings under drought stress by RP—HPLC |
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| DOI：10.7606/j.issn.1000-7601.2009.01.37 |
| 中文关键词: 小麦幼苗 水分胁迫 甜菜碱 反相高效液相色谱法 |
| 英文关键词:wheat weedings drought stress betaine reverse phase HPLC |
| 基金项目:国家自然科学基金资助项目(30671253)； 中国博士后科学基金资助项目 |
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| 中文摘要: |
| 采用胁迫及复水处理不同品种小麦幼苗,甜菜碱提取液经浓缩后分别在阴阳离子交换树脂上纯化,样品用C18反相色谱柱进行分离,流动相为50 mmol/L的NaH2PO4溶液(pH4.45),流速为0.6 mL/min,192 nm波长测定。实验优化了提取温度、时间、色谱离子对等相关影响因素,测定了水分胁迫下小麦幼苗叶片中甜菜碱含量的变化。研究结果发现:在PEG胁迫和复水过程中,随时间的延长,小麦幼苗中的甜菜碱的含量均呈现先增后降趋势,抗旱型小麦能在较短时间内迅速积累大量甜菜碱;甜菜碱的合成与植物抗旱有着密切关系。反相高效液相色谱法测定小麦甜菜碱含量具有准确、灵敏、简便等特点。 |
| 英文摘要: |
| We used the different wheat seedings which were planted on the condition that water is restricted and resupply.The concentrated betain samples were purified by passing through the ion-exchange resin.We used the Reverse-Phase High Performance Liquid Chromatography technology to determine the change of betaine content.In the HPLC system, the buffer is 0.05 M sodium phosphate (pH4.45) , the column was kept at 28℃ and the flow rate was 0.6 mL/min, the samples was determined at 192 nm wavelength.Besides, we optimized the factors, such as temperature, time and ion pair.During both the PEG-induced drought stress and water resupplied process, the amount of betaine increased at first and reduced latter.A great deal of Betaine accumulated in the anti-drought type wheat in a short time.This result showed that the synthesis of betaine have close connection with anti-drought plant.This method was exact, sensitive and convenient, as well provided a basis for anti-drought mechanism and the wheat breeding work. |
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