邱睿,王兆,王保莉,曲东.干旱胁迫下硫对小麦叶片GSH 含量及GSH—PX 活性的影响[J].干旱地区农业研究,2009,27(2):148~152
干旱胁迫下硫对小麦叶片GSH 含量及GSH—PX 活性的影响
Effect of sulphur on the content of GSH and the activity of GSH—PX in wheat leave under drought stress
  
DOI：10.7606/j.issn.1000-7601.2009.02.27
中文关键词:  硫  干旱胁迫  谷胱甘肽  谷胱甘肽过氧化酶
英文关键词:sulphur  drought stress  GSH  GSH-PX
基金项目:国家大学生创新性实验计划(G2007046)
作者单位
邱睿 College of Life Sciences Northwest A＆F University,Yangling,Shaanxi 712100,China 
王兆 College of Life Sciences Northwest A＆F University,Yangling,Shaanxi 712100,China 
王保莉 College of Life Sciences Northwest A＆F University,Yangling,Shaanxi 712100,China 
曲东 College of Resources and Environment Northwest A＆F University,Yangling,Shaanxi 712100,China 
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中文摘要:
      生物体内的谷胱甘肽对消除逆境产生的活性氧具有重要的作用。无机硫营养吸收与体内有机硫的转化有着密切相关,研究干旱胁迫下硫营养与谷胱甘肽(GSH)含量及谷胱甘肽过氧化酶(GSH-PX)活性的关系,对揭示硫素的抗逆作用机理具有重要的理论和实际意义。本研究以高水肥型小麦品种郑引1号和抗旱型品种陕合6号为供试材料,分别用Hoagland全营养液和缺硫营养液进行溶液培养试验,探讨了模拟干旱胁迫过程下小麦苗期功能叶片中GSH含量和GSH-PX活性的变化。结果表明:陕合6号的GSH含量显著高于郑引1号;水分胁迫下,郑引1号中供硫处理的GSH含量上升迅速,24 h、48 h及72 h分别比供水处理增加了26.4%、33.5%和56.6%,而缺硫处理则分别是供水处理的93.2%、95.9%和74.5%;陕合6号在水分胁迫的72 h内供硫处理的GSH含量变化不明显,分别为供水处理98.3%106.1%,而缺硫处理的GSH含量有明显的增加,分别比供水处理增加了13.1%、40.2%和45.9%。郑引1号的GSH含量受水分胁迫和硫营养供给状况的影响显著,而陕合6号的GSH含量仅在缺硫情况下对水分胁迫敏感。水分胁迫初期(24 h)可诱导GSH-PX活性增高,郑引1号供硫处理较供水处理增加了23.9%,而缺硫处理仅增加了7.4%;陕合6号品种的供硫处理和缺硫处理分别较供水处理增加了24.5%和16.8%。随着干旱胁迫时间的延长(4 872 h),GSH-PX活性均呈降低趋势,但供硫处理的GSH-PX活性始终高于缺硫处理。郑引1号高水肥型品种的GSH-PX活性较低,在缺硫环境下受水分胁迫的诱导不敏感;而陕合6号抗旱型品种的GSH-PX活性整体上较高,并且对水分胁迫的响应比较敏感。
英文摘要:
      Glutathione (GSH) in organism may play an important role on elimination of reactive Oxygen species produced under stress.There are close relationship between absorbation of inorganic sulphur and transformation/assimilation of organic sulphur in organism.Research on effect of sulphur on the content of GSH and the activity of gutathione peroxidase (GSH-PX) in wheat leave under drought stress may have a greatly significance for illustrating resisting stress mechanism of sulphur nutrition.The content of GSH and the activity of GSH-PX of the seedling of drought-sensitive wheat strain Zhengyin No.1 and drought-tolerant strain Shanhe No.6 cultured in complete Hoagland solution containing sulphur and Hoagland solution without sulphur were compared.The results showed the content of GSH of drought-tolerant wheat was clearly higher than the drought-sensitive wheat.Under drought stress, the GSH content in Zhengyin No.1 with the sulphur-lack treatment were rised rapidly, in which increasing 26.4%, 33.5% and 56.6% compare to Shanhe No.6, at 24 h, 48 h and 72 h of drought stress, respectively.For Shanhe No.6, the chenges of GSH content during 72 h drought stress with the treatment of sulphur-rich were unconspicuous, which was 98.3% to 106.1% under providing water, but the one with the treatment of sulphur-lack rised conspicuously, was 13.1%, 40.2% and 45.9% than under providing water.The GSH content for Zhengyin No.1 was remarkable impressed by drought stress and suppplyment of sulphur nutrition, but for Shanhe No.6, was only sensitively to drought stress under the sulphur-lack.At the initial stages (24 h) , the GSH-PX activities were increased. For Zhengyin No.1, GSH-PX activities under the treatment of sulphur-rich were 23.9% higher than under the water-providing treatment, but it was only 7.4% under the treatment of sulphur-lack higher than the treatment of water-providing.;for Shanhe No.6, GSH-PX activities were respectively 24.5% under the treatment of sulphur-rich and 16.8% under the treatment of sulphur-lack higher than the treatment of water-providing, respectively.As the prolong of the drought stress, the activities of GSH-PX were all fall down, but under the treatment of sulphur-rich it was always higher than the treatment of sulphur-lack.For Zhengyin No.1, the activities of GSH-PX level were lower, and unsensitively to drought stress under the sulphur-lack, however, for Shanhe No.6, it was all higher, and sensitively to drought stress.
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