| 范小峰,杨颖丽,程转霞.NaCl胁迫下唐古特白刺愈伤组织生理生化变化研究[J].干旱地区农业研究,2009,27(3):203~207 |
| NaCl胁迫下唐古特白刺愈伤组织生理生化变化研究 |
| Research on physiological and biochemical index changes of Nitraria tangutorm Bobr．callus under NaCl stress |
| |
| DOI：10.7606/j.issn.1000-7601.2009.03.38 |
| 中文关键词: 盐胁迫 唐古特白刺 愈伤组织 生长 生理生化指标 |
| 英文关键词:salt stress Nitraria tangutorm Bobr. callus growth physiological and biochemical index |
| 基金项目:甘肃省科技攻关计划项目(2GS035-A41-001-06)； 甘肃省教育厅研究生导师科研项目(0701-23) |
|
| 摘要点击次数: 482 |
| 全文下载次数: 283 |
| 中文摘要: |
| 以唐古特白刺愈伤组织为材料,研究NaCl胁迫对细胞生长、可溶性蛋白、丙二醛、游离脯氨酸含量、SOD、POD、CAT活性等的影响,探讨唐古特白刺抵抗NaCl胁迫的生理机制。结果表明:游离脯氨酸、丙二醛含量随NaCl浓度的提高和胁迫时间的延长而增加;不同浓度NaCl胁迫下,过氧化物酶(POD)活性随处理时间呈先升后降的变化;超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、可溶性蛋白含量、愈伤组织生长量在低盐胁迫下,随着培养时间的延长,均呈上升趋势,而在高盐胁迫下,随着培养时间的延长,呈先升后降的变化。由此表明,唐古特白刺具有一定适应盐渍生境的能力,轻度盐胁迫有利于白刺愈伤组织的生长。 |
| 英文摘要: |
| Callus of Nitraria tangutorm Bobr.were used to investigate the effects of NaCl stress on growth, content of soluble protein, MDA, proline and activities of SOD, POD and CAT, in order to understand the physiological mechanism of Nitraria tangutorm Bobr.callus resistance to NaCl stress at cell level.The results indicated that free Proline and MDA content increased as NaCl concentration enhanced and stress time lengthened;the activities of Peroxidase (POD) first increased and then decreased as time lengthened under different NaCl concentration;the activities of superoxide dismutase (SOD) , catalase (CAT) , soluble protein and callus growth expressed increasing trend as time lengthened in low NaCl;but in high NaCl, they first increased and then decreased as time lengthened.It follows that N.tangutorm had a adaptability to salt environment, light salt stress is favorable for N.tangutorm callus to grow. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| | |