赵映琴,刘玉汇,王丽,杨宏羽,张俊莲,王蒂.低磷胁迫下马铃薯试管苗生长及生理指标变化研究[J].干旱地区农业研究,2009,27(5):183~187
低磷胁迫下马铃薯试管苗生长及生理指标变化研究
Study on the growth and changes of several physiological indexes of potato plantlets in vitro under low phosphorus stress
  
DOI：10.7606/j.issn.1000-7601.2009.05.33
中文关键词:  马铃薯  试管苗  低磷胁迫  生理指标
英文关键词:potato  plantlet in Vitro  low-phosphorus stress  physiological index
基金项目:国家科技支撑计划项目(2006BAD21B05)； 农业部行业专项项目(nyhyzx-07-006-3)； 甘肃省援藏项目(0708JKCA063)
作者单位
赵映琴 College of Agronomy Gansu Agricultural University, Lanzhou, Gansu 730070, China 
刘玉汇 Gansu Key Laboratory of Crop Genetic ＆Germplasm Enhancement, Lanzhou, Gansu 730070, China 
王丽 Gansu Key Laboratory of Crop Genetic ＆Germplasm Enhancement, Lanzhou, Gansu 730070, China；College of Life Sciences and Technology Gansu Agricultural University, Lanzhou, Gansu 730070, China 
杨宏羽 College of Agronomy Gansu Agricultural University, Lanzhou, Gansu 730070, China；Gansu Key Laboratory of Crop Genetic ＆Germplasm Enhancement, Lanzhou, Gansu 730070, China 
张俊莲 College of Agronomy Gansu Agricultural University, Lanzhou, Gansu 730070, China；Gansu Key Laboratory of Crop Genetic ＆Germplasm Enhancement, Lanzhou, Gansu 730070, China 
王蒂 College of Agronomy Gansu Agricultural University, Lanzhou, Gansu 730070, China；Gansu Key Laboratory of Crop Genetic ＆Germplasm Enhancement, Lanzhou, Gansu 730070, China 
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中文摘要:
      马铃薯试管苗在全价、60%磷素和30%磷素的MS培养基上培养,第20 d、40 d时测定生理指标。结果表明,低磷胁迫对马铃薯试管苗新叶和侧根发生以及根生长具有促进作用,且地上部和地下部鲜重小幅增加。30%低磷培养环境下,试管苗的细胞膜透性值、可溶性蛋白质和可溶性糖含量均较对照明显增加,而叶绿素含量则明显降低,差异达到极显著或显著水平。60%低磷处理下,这些指标的变化与对照基本相同,差异不显著,表明含60%P元素的MS培养基适宜马铃薯试管苗生长。
英文摘要:
      Potato plantlets in vitro were cultured on MS medium with full phosphorus level, 60% phosphorus level and 30% phosphorus level.Physiological indexes on 20 d and 40 d were determined and the results showed that the ability of new leaves and lateral root generation was promoted under low-p stress, which could also improve the root growth, increase the fresh weight of aboveground and underground slightly.Under the culture environment with 30% phosphorus, the relative permeability of cell membrane, soluble protein content and soluble sugar content increased and chlorophyll content decreased significantly compared with the control, and the significant difference was at the level of 5% or 1%.Under the treatment with 60% phosphorus, the changes of physiological indexes were the same as the control, and there was no obvious difference.The results demonstrated that it is suitable for potato plantlets in vitro growth on MS medium with 60% phosphorus.
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