| 刘建新,王鑫,李博萍.外源一氧化氮供体SNP 对NaCl 胁迫下多裂骆驼蓬幼苗氧化损伤的保护效应[J].干旱地区农业研究,2009,27(6):139~143 |
| 外源一氧化氮供体SNP 对NaCl 胁迫下多裂骆驼蓬幼苗氧化损伤的保护效应 |
| Effects of exogenous nitric oxide donor SNP on protecting Peganum multisectum seedlings from oxidative damage under NaCl stress |
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| DOI：10.7606/j.issn.1000-7601.2009.06.27 |
| 中文关键词: 多裂骆驼蓬 一氧化氮 NaCl胁迫 氧化损伤 |
| 英文关键词:Peganum multisectum Bobr nitric oxide NaCl stress oxidative damage |
| 基金项目:甘肃省教育厅科研基金资助项目(JY0510-07) |
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| 中文摘要: |
| 研究了外源一氧化氮(NO)供体硝普钠(SNP)对NaCl胁迫下多裂骆驼蓬幼苗生长和氧化损伤的影响。结果表明:正常生长条件下喷施SNP能促进多裂骆驼蓬幼苗的生长,而NO信号传递途径关键酶鸟苷酸环化酶(GC)抑制剂亚甲基蓝(MB)明显抑制幼苗的生长;喷施SNP预处理显著缓解了NaCl胁迫对幼苗生长的抑制,明显降低了NaCl胁迫下叶片丙二醛(MDA)、超氧阴离子(O2.)和H2O2的积累及过氧化氢酶(CAT)活性,提高了超氧化物歧化酶(SOD)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性;使抗坏血酸(ASA)和谷胱甘肽(GSH)含量及(Spd+Spm)/Put比值显著提高,而MB逆转了SNP对NaCl胁迫幼苗生长的促进作用及MDA、H2O2、CAT、POD、ASA、GSH和(Spd+Spm)/Put的调节作用,但对SNP调节O-2、SOD和APX的效应无明显影响。表明外源NO可能通过GC介导的cGMP依赖和非cGMP依赖两条途径参与盐胁迫下活性氧代谢的调节,从而缓解盐胁迫导致的氧化损伤,促进多裂骆驼蓬幼苗的生长。 |
| 英文摘要: |
| Effects of exogenous nitric oxide(NO) donor sodium nitroprusside(SNP) on growth of Peganum multisectum Bobr seedlings and protecting them from oxidative damage under salt stress were investigated.The results indicated that spraying SNP on leaves increased the growth of P.multisectum seedlings in normal growth conditions,but exogenous methylene blue,the inhibitor of the key enzyme guanylyl cyclase during signal transfer of NO,decreased significantly the growth of P.multisectum seedlings.Spraying SNP pretreatment alleviated obviously the inhibition of P.multisectum seedlings growth under NaCl stress,the contents of malondiadehyde(MDA) and H2O2,the O-2 producing rate and catalase(CAT) active were decreased while the activities of superoxide dismutase(SOD),peroxidase(POD) and ascorbate peroxidase(APX),the contents of ascorbate acid(ASA) and reduced glutathione(GSH),and(Spd+Spm)/Put ratio were significantly increased in P.multisectum leaves under NaCl stress.But methylene blue reversed some effects of exogenous SNP under NaCl stress,such as the plant biomass amount and POD activity,the contents of ASA and GSH,(Spd+Spm)/Put were decreased,the contents of MDA and H2O2 and CAT activity were significantly increased,however,the O2· producing rate and the activities of SOD and APX were not markedly affected under NaCl stress.These results suggested that NO might involve in regulation of reactive oxygen species metabolism through cyclic guanosine monophosphate(cGMP)-dependent and cGMP-independent pathways to alleviate the oxidative damage and the inhibition of P.multisectum seedlings growth under salt stress. |
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