| 华智锐,马锋旺,李小玲,梁东.百合转S6PDH基因植株的抗盐性鉴定[J].干旱地区农业研究,2010,28(1):160~163 |
| 百合转S6PDH基因植株的抗盐性鉴定 |
| Salt-resistance identification of transformed Lilium with S6PDH gene |
| |
| DOI：10.7606/j.issn.1000-7601.2010.01.29 |
| 中文关键词: 百合 S6PDH基因 抗盐性 生理指标 |
| 英文关键词:lily S6PDH gene salt resistance physiological indicator |
| 基金项目:商洛学院科研基金项目（07SKY024） |
|
| 摘要点击次数: 444 |
| 全文下载次数: 274 |
| 中文摘要: |
| 对农杆菌介导S6PDH基因导入麝香百合的转化植株和对照进行盐胁迫处理，通过测定相关生理指标，对其抗盐性进行评价。研究结果表明：随着盐胁迫（10 g/L NaCl）时间延长，转化苗叶片与对照的电导率和MDA含量均呈上升趋势，可溶性蛋白含量均下降，同时SOD、POD、CAT、APX酶活性均呈先升后降趋势；但在整个胁迫过程中，转化苗电导率和MDA含量均小于对照，可溶性蛋白含量比对照高，且具有比对照更高的酶活性，通过差异显著性分析，差异达显著水平。说明转化苗已经明显表现出了较强的抗盐性。 |
| 英文摘要: |
| Based on Agrobacterium-mediated S6PDH gene being delivered into Lilium longiflorum, by treatment of salt stress to the transformed plants and the control, some related physiological indicators were measured for a short period of time to lay foundation for improving resistant varieties of lily and studying the resistant mechanism of transgenic plants. The main findings are as following: With salt stress (10 g／L NaCl) to extend the time, leaves
of the conductivity and MDA of transformed plants and the control showed an upward trend, and soluble protein content decreased, while the activity of SOD, POD, CAT and APX rose first, then fell down. But in the process of salt stress, the conductivity and MDA of transformed seedlings were less than that of the control, while soluble protein content was higher than that of the control, and had higher activity than non-transformed seedlings. The analysis showed that the difference reached a significant level, and the transformed seedlings demonstrated o
bviously strong salt-resistance. |
| 查看全文 查看/发表评论 下载PDF阅读器 |