| 曹君迈,祁金涛,刘建利,闫兴富,李满.甘草不同来源愈伤组织过氧化物酶分析[J].干旱地区农业研究,2010,28(4):123~127 |
| 甘草不同来源愈伤组织过氧化物酶分析 |
| Analysis of peroxidase isoenzymes from different callus of Glycyrrhiza uralensis |
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| DOI：10.7606/j.issn.1000-7601.2010.04.23 |
| 中文关键词: 甘草 愈伤组织 过氧化物酶 |
| 英文关键词:Glycrrhiza uralensis callus peroxidase isoenzyme |
| 基金项目:宁夏自然科学基金“甘草脱分化过程中细胞结构及生理变化的研究”(NZ0953) |
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| 中文摘要: |
| 采用聚丙烯酰胺凝胶电泳法和分光光度法,对乌拉尔甘草的根状茎、芽、叶诱导的愈伤组织中过氧化物同功酶进行电泳和活性分析。结果得出:不同来源的愈伤组织中过氧化物酶酶谱分为A、B、C三个区域,其中活性最强的是A区和C区,所有供试样品出现3种酶谱谱型,6种酶谱谱带;在进行酶活性测定时,叶的愈伤组织中过氧化物酶活性最高,芽的次之,根状茎的最低,过氧化物酶活性过高不利于愈伤组织生长。确立的2种特征酶谱带(P2和P6),4种差异酶谱谱带(P1、P3、P4和P5),为乌拉尔甘草种的鉴别及脱分化与再分化的细胞生物学机制研究提供了一定的参考依据。 |
| 英文摘要: |
| The electrophoretic patterns of peroxidase isoenzymes and peroxidase activity of callus inducted from the
rhizome, bud and leaf of Glycyrrhiza uralensis were analyzed with PAGE and spectrophotometry. The results demonstrated that the electrophoretic pattens of peroxidase isoenzymes from different callus were divided into A, B and C regions, in which the regions of A and C were the most active. All had 3 kinds of patterns and 6 bands. Meanwhile, the order of peroxidase activity from deferent callus were leaf >buds>rhizome. But too high peroxidase activity was not beneficial to the development of callus. P2 and P6 were common bands in 3 kinds of explants, while P1, P3，P4 and P5 were differ-ent bands. These could be used to identify Glycyrhiza uralensis from other species and to study the diferentiation and redifferentiation of Glycyrrhiza uralensis. |
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