| 薛清,王玲娜,段春梅,林雁冰.CaCl2对干旱地区钙质土壤放线菌分离效果的影响[J].干旱地区农业研究,2010,28(5):104~107 |
| CaCl2对干旱地区钙质土壤放线菌分离效果的影响 |
| The influence of adding CaCl2 on the isolation of actinomycetes from calcareous soil in arid areas |
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| DOI：10.7606/j.issn.1000-7601.2010.05.19 |
| 中文关键词: 拮抗性放线菌 分离 CaCl2 钙质土壤 干旱区 |
| 英文关键词:antagonistical actinomycetes isolation CaCl2 calcareous soil arid areas |
| 基金项目:国家自然科学基金项目（40701095，40971171）；西北农林科技大学“大学生创新实验计划项目”(2008021) |
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| 中文摘要: |
| 采用稀释平板法研究了高氏1号培养基中加入CaCl2对钙质土壤放线菌分离效果的影响。结果表明：（1） 加入CaCl2对干旱区钙质土壤放线菌的出菌量有一定影响，大部分土壤的放线菌总数较对照增长23.9%～47.0%，加钙处理与对照的差异达到显著水平（P＜0.05）；加钙后链霉菌数量有增有减，增、减幅度分别为17.5%～123.3%、18.0%～
42.5%，减少幅度未达到显著水平（P＞0.05）。(2) 向高氏1号培养基中加钙有利于广谱高效拮抗
性放线菌的分离。 |
| 英文摘要: |
| Dilution plate method was employed to study the effects of adding CaCl2 into Gause's No.1 medium on the isolation of actinomycetes from calcareous soil. The result showed that: (1) The total quantity of the actinomycetes isolated from calcareous soil in arid areas could be affected by CaCl2. The total quantity of the actinomycetes was increased from 23.9% to 47.0% mostly. The difference between treatments and the control was significant at 5% level. The effect on the total quantity of the streptomycete was different from that of actinomycetes. The increase range was from 17.5% to 123.3%, while the decrease range was from 18.0% to 42.5% compared with the control. But the decrease wasn't significant at 5% level (P＞0.05). (2) Adding CaCl2 into Gause's No.1 medium increased the quantity of broad-spectrum with strong antimicrobial antinomycetes. |
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