小青菜酶活性与铅形态关系的研究

殷宪强; 王昌钊; 易磊; 刘永轩; 王国栋; 张兴昌

殷宪强,王昌钊,易磊,刘永轩,王国栋,张兴昌.小青菜酶活性与铅形态关系的研究[J].干旱地区农业研究,2010,28(5):133~137

小青菜酶活性与铅形态关系的研究

Study on relationship between Pb chemical forms and enzyme activity in Brassica Chinensis

DOI：10.7606/j.issn.1000-7601.2010.05.25

中文关键词: 小青菜铅形态植物酶活性

英文关键词:Brassica Chinensis Pb chemical form plant enzyme activity

基金项目:中科院知识创新工程重要方向(KZCX2-YW-441);国家973计划(2007CB106803)；西北农林科技大学科研专项（07ZR044）

作者	单位
殷宪强	西北农林科技大学资源环境学院，陕西杨凌 712100
王昌钊	陕西出入境检验检疫局，陕西西安 710068
易磊	西北农林科技大学资源环境学院，陕西杨凌 712100
刘永轩	西北农林科技大学资源环境学院，陕西杨凌 712100
王国栋	西北农林科技大学资源环境学院，陕西杨凌 712100
张兴昌	西北农林科技大学资源环境学院，陕西杨凌 712100；西北农林科技大学/中国科学院水利部水土保持研究所，陕西杨凌 712100

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中文摘要:

采用添加外源铅和室内培养的方法，研究了黄绵土盆栽小青菜体内铅的形态分布规律及铅形态对植物酶活性的影响。结果表明：土壤受铅污染后小青菜茎叶不同形态的铅的呈显著和极显著增加，根部各形态铅含量随土壤中外源Pb含量的增加呈极显著增加，铅污染后小青菜体内铅活性提高。在外源Pb作用下小青菜茎叶内多酚氧化酶和过氧化氢酶的活性显著提高，以保护植物免受铅的危害，同时可以指示植物体受到高浓度铅的危害，保护酶系统的变化主要受醋酸提取态和盐酸提取态铅的影响。

英文摘要:

This study investigated the Pb chemical forms distribution and the effect of Pb forms on plant enzyme activity of Brassica Chinensis indoor cultivated with added Pb in loessal soil. The result showed that the Pb concentration of differenct forms in shoot/leaf of Brassica Chinensis increased significantly and extremely significantly, and all Pb forms in root extremely significantly increased with Pb increasing after the soil was polluted by Pb. The acativity of Pb in Brassica Chinensis increased after the soil was polluted by Pb. In order to erase the hazards of Pb to Brassica Chinensis, the activity of polyphenol oxida se and catalase increase significantly, which also can indicate the hazards of Pb to plant. Among all Pb forms HAc-extractable Pb and HCl-extractable Pb was the dominant harmful forms to the protection enzyme system in Brassica Chinensis.

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