根瘤菌的RAPD分子标记技术检测和耐盐碱筛选

陈利云; 张海林; 周志宇

陈利云,张海林,周志宇.根瘤菌的RAPD分子标记技术检测和耐盐碱筛选[J].干旱地区农业研究,2010,28(6):212~216

根瘤菌的RAPD分子标记技术检测和耐盐碱筛选

Technical detection of RAPD molecule maker and saline alkali tolerance experiments on Rhizobia

DOI：10.7606/j.issn.1000-7601.2010.06.38

中文关键词: 根瘤菌 RAPD技术筛选试验耐盐碱

英文关键词:Rhizobium RAPD screening test salt and alkali tolerance

基金项目:国家重点基础研究发展规划项目(973项目)(2007CB108903)

作者	单位
陈利云	天水师范学院生命科学与化学学院,甘肃天水 741000
张海林	天水师范学院生命科学与化学学院,甘肃天水 741000
周志宇	兰州大学草地农业科技学院,甘肃兰州 730020

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中文摘要:

采用17株分离于苜蓿和草木樨的根瘤菌为研究对象,进行了RAPD分子标记技术检测和耐盐碱筛选实验。结果显示:供试根瘤菌菌体基因组RAPD电泳图谱分为6组不同的条带,说明17株菌中有6个不同菌系。不同菌系的根瘤菌耐盐碱筛选试验表明,来自临泽的根瘤菌耐盐碱能力均比来自天水的强,其中两株能在pH14的YMA培养基上生长,一株可耐1.539 mol/L的NaCl,两株能耐1.971 mol/L的Na₂SO₄,尤其菌GL007对高pH和两种盐都有较强的忍耐性,是实验筛选出的珍贵耐盐碱菌种资源。

英文摘要:

With 17 Rhizobia strains isolated from nodules of alfalfa and sweet clover as the research objects, RAPD detection and salt and alkali tolerance screening tests were conducted. The results showed that there were 6 different bac-terial systems in 17 Rhizobia. The experiment of salt and alkali tolerance showed that rhizobia of alfalfa in Linze had bet-ter stress tolerance ability than that in Tianshui. Two strains could grow well under pH 14, one strain could grow at medi-um containing 9% NaCl and two strains could tolerate 1.971 mol/L Na₂SO₄ on YMA medium. All these data showed GL007 strain had strong tolerance ability for salt and high pH. It was a precious strain resource of saline- alkali tolerance.

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