| 刘建利,曹君迈,马文平.宁夏乌拉尔甘草内生菌鉴定及甜瓜采后病害病原拮抗菌的筛选[J].干旱地区农业研究,2011,29(1):252~256 |
| 宁夏乌拉尔甘草内生菌鉴定及甜瓜采后病害病原拮抗菌的筛选 |
| Identification of endophytes from Glycyrrhiza uralensis in Ningxia and screening of antagonism against pathogens of post-harvest muskmelon |
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| DOI：10.7606/j.issn.1000-7601.2011.01.46 |
| 中文关键词: 乌拉尔甘草 内生菌 甜瓜采后病害病原菌 筛选 |
| 英文关键词:Glycyrrhiza uralensis endophyte identification pathogens of post-harvest nuskmelon selection |
| 基金项目:宁夏回族自治区高等学校科学研究项目（2009JY011） |
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| 中文摘要: |
| 采用组织分离法从宁夏地区乌拉尔甘草的根、茎、叶中分离内生菌，依据形态进行初步鉴定；以甜瓜采后病害病原菌为供试菌株，测定甘草内生细菌抑菌活性。结果显示：从宁夏地区乌拉尔甘草的根、茎、叶中共分离出内生菌287株，根中分离出142株，茎中分离出78株，叶中分离出67株,136株属内生真菌，151株属内生细菌。对产孢的53株内生真菌进行初步鉴定，分类为2纲4科6属，链格孢属（Alternaria）、曲霉属（Aspergillus）和青霉属（Penicillium）为优势种群。151株内生细菌中，革兰氏阳性菌、杆菌占优势。其中8株内生细菌对4种甜瓜采后病害病原菌的抑制能力均较强。 |
| 英文摘要: |
| The study was aimed at solating and identifying endophytes from Glycyrrhiza uralensis and detecting antimicrobial activity of these strains. The endophytes was isolated by using tissue isolation method from the root, stem and leaf of Glycyrrhiza uralensis in Ningxia and identified preliminarily based on morphological characteristics. With the pathogens of post-harvest muskmelon as test fungi，the antimicrobial activity of endophytic bacteria was investigated. The results showed that 287 endophytes were isolated from the roots, stems and leaves of Glycyrrhiza uralensis in Ningxia respectively, of which 142 were from roots, 78 from stems and 67 from leaves, and 151 belonged to bacteria and 131 to fungi. The 53 spore-producing fungi were classfied into 6 genera under 4 families and 2 orders, e.g Penicillium, Alternaria and Aspergillus were dominant. G+ and Baci
llus were dominant in endophytic bacteria. And 8 endophytic bacteria with higher activity against all pathogens of post harvest muskmelon were selected out. |
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