杨宏新,毛培春,孟林,呼天明,高洪文.19份高燕麦草种质材料苗期抗旱性评价[J].干旱地区农业研究,2011,29(2):6~14
19份高燕麦草种质材料苗期抗旱性评价
Assessment of drought resistance for 19 germplasm and materials of Arrhenatherum elatius at the seedling stage
  
DOI：10.7606/j.issn.1000-7601.2011.02.02
中文关键词:  高燕麦草  苗期  抗旱性  评价
英文关键词:Arrhenatherum elatius(L.) J. et C. Pressl  seedling stage  drought resistance  assessment
基金项目:国际科技合作与交流项目(2008DFR30200)；北京市科技计划项目(Z09090501040902)；国家牧草种质资源保护项目；北京市农林科学院常规育种财政专项
作者单位
杨宏新 西北农林科技大学动物科技学院,陕西 杨凌 712100
北京市农林科学院北京草业与环境研究发展中心,北京 100097 
毛培春 北京市农林科学院北京草业与环境研究发展中心,北京 100097 
孟林 北京市农林科学院北京草业与环境研究发展中心,北京 100097 
呼天明 西北农林科技大学动物科技学院,陕西 杨凌 712100 
高洪文 中国农业科学院北京畜牧兽医研究所,北京 100193 
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中文摘要:
      将从11个国家收集到的19份高燕麦草(Arrhenatherum elatius(L.)J.et C.Pressl)种质材料,置于温室采用苗期模拟干旱胁迫-复水法进行抗旱性评价试验。于停水当天(CK)、持续干旱胁迫7、14、21、28、354、2 d和复水后7 d分别取样测定叶片的相对含水量(RWC)、相对电导率(REC)、脯氨酸含量(Pro)、丙二醛含量(MDA)、可溶性糖含量(SSC)、超氧化物歧化酶(SOD)、存活率(SR)、根冠比(RSR)8个抗旱指标,综合评价其抗旱性。结果显示,以持续干旱胁迫到42 d时上述8个指标测定值的离差平方和—平方欧式距离法综合聚类分析结果为依据,可将19份种质材料划分为三个抗旱等级。其中抗旱性较强的包括ZXY2005P-1021、ZXY2005P-619、ZXY2005P-853、ZXY2005P-1160、ZXY2005P-1319、ZXY2005P-901;抗旱性中等的包括ZXY2005P-706、ZXY2005P-1086、ZXY2005P-1473、ZXY2005P-1296、ZXY2005P-1182、ZXY2005P-1036、ZXY2005P-877、ZXY2005P-837;抗旱性较弱的包括ZXY2005P-1514、ZXY2005P-969、ZXY2005P-1375、ZXY2005P-1362、ZXY2005P-1426。同时,随着干旱胁迫时间的延续,叶片RWC呈下降趋势,REC、Pro、SSC则显著上升,SOD、MDA虽有波动但整体呈上升趋势。复水7 d后,叶片RWC、Pro均可恢复到胁迫前的水平,而REC、SSC、MDA、SOD值虽有不同程度降低但均未恢复到胁迫前的水平。
英文摘要:
      With the method of simulating drought stress-water recovery in greenhouse, 8 drought resistant indexes , including the leaf relative water content(RWC), relative electrical conductivity (REC)，free proline accumulation (Pro), soluble sugar content (SSC)，superoxide dismutase(SOD), malondialdehyde (MDA), survival rate (SR), root-shoot ratio (RSR) on the 0 d(CK), 7th d, 14th d, 28th d, 35th d and 42th d under water stress and the 7th d after rewatering, respectively, of 19 Arrhenatherum elatiusm germplasm and materials at the seedling stage were measured and analyzed in order to assess comprehensively the drought resistance. The values of the eight indexes which has been treated under the condition of 42 d of resistant drought stress, which has been analyzed by the Wared's method and Squared Euclidean dis-tance cluster analysis, the results showed that three drought resistant classes including the higher drought resistance (in- cluding the germplasm of ZXY2005P-1021, ZXY2005P-619, ZXY2005P- 853, ZXY2005P-1160, ZXY2005P-1319，ZXY200SP-901), the medium drought resistance ( including ZXY2005P-706，ZXY2005P-1086, ZXY2005P-1473, ZXY2005P-1296, ZXY2005P-1182, ZXY2005P-1036, ZXY2005P-877, ZXY2005P-837) and the weaker drought resistance (ZXY2005P-1514, ZXY2005P-969，ZXY2005P-1375, ZXY2005P-1362, ZXY2005P-1426) were classified. And the leaf RWC of 19 germplasm decreased, the SSC, Pro and REC increased but the SOD and MDA increased with volatility while the drought stress days were increased. Seven days after rewatering, the RWC and Pro were restored to pristine level, but the SOD and MDA were not restored to the level before stress were exerted al-though they declined in varying degrees.
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