水稻脯氨酸代谢关键酶对水分胁迫的响应

赵贵林; 陈强; 胡国霞; 褚妍; 马莲菊; 李雪梅

赵贵林,陈强,胡国霞,褚妍,马莲菊,李雪梅.水稻脯氨酸代谢关键酶对水分胁迫的响应[J].干旱地区农业研究,2011,29(3):80~83

水稻脯氨酸代谢关键酶对水分胁迫的响应

Responses of the key enzymes involved in proline metabolism in rice seedling under water stress

DOI：10.7606/j.issn.1000-7601.2011.03.15

中文关键词: 水分胁迫脯氨酸吡咯啉-5-羧酸合成酶鸟氨酸转氨酶脯氨酸脱氢酶

英文关键词:water stress proline △¹-pryroline-5-carboxylate synthetase (P5CS) orithine-δ-aminotransferase (δ-OAT) proline dehydrogenase(ProDH)

基金项目:国家自然科学基金资助课题(30870205；31070285)；辽宁省教育厅资助课题(L2010516)；辽宁省自然科学基金(20092070,20102205)；沈阳师范大学博士后资助课题

作者	单位
赵贵林	College of Chemical and Life Sciences, Shenyang Normal University, Shenyang 110034,China
陈强	College of Chemical and Life Sciences, Shenyang Normal University, Shenyang 110034,China
胡国霞	College of Chemical and Life Sciences, Shenyang Normal University, Shenyang 110034,China
褚妍	College of Chemical and Life Sciences, Shenyang Normal University, Shenyang 110034,China
马莲菊	College of Chemical and Life Sciences, Shenyang Normal University, Shenyang 110034,China
李雪梅	College of Chemical and Life Sciences, Shenyang Normal University, Shenyang 110034,China

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中文摘要:

以二叶期水稻幼苗为试验材料,研究其在15%PEG6000模拟的水分胁迫下脯氨酸积累及代谢关键酶活性变化,结果表明:叶片和根系中脯氨酸含量在胁迫后快速积累,胁迫24 h达到最大值,随后逐渐降低;控制脯氨酸合成的关键酶吡咯啉-5-羧酸合成酶(P5CS)和鸟氨酸转氨酶(δ-OAT)活性都呈现先升后降的变化趋势,而控制脯氨酸降解的关键酶脯氨酸脱氢酶(ProDH)活性呈现先降后升的变化趋势。

英文摘要:

Using rice seedlings at two-leaf stage as tested materials, the free proline content and activities of en-zymes involved in proline metabolism were studied under water stress induced by 15%PEG. The results showed that the proline content increased obviously under stress, reached the maximum after 24 h and then decreased. The activities of △¹-pyroline-5-carboxylate synthetase (P5CS ) and ornithine- δ-aminotransferase (δ-0AT)， the key enzymes of proline accumulation, increased sigificantly and then declined; while the activity of proline dehydrogenase (ProDH)， a key en-zyme of proline degradation, declined significantly and then increased.

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