| 赵贵林,陈强,胡国霞,褚妍,马莲菊,李雪梅.水稻脯氨酸代谢关键酶对水分胁迫的响应[J].干旱地区农业研究,2011,29(3):80~83 |
| 水稻脯氨酸代谢关键酶对水分胁迫的响应 |
| Responses of the key enzymes involved in proline metabolism in rice seedling under water stress |
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| DOI：10.7606/j.issn.1000-7601.2011.03.15 |
| 中文关键词: 水分胁迫 脯氨酸 吡咯啉-5-羧酸合成酶 鸟氨酸转氨酶 脯氨酸脱氢酶 |
| 英文关键词:water stress proline △1-pryroline-5-carboxylate synthetase (P5CS) orithine-δ-aminotransferase (δ-OAT) proline dehydrogenase(ProDH) |
| 基金项目:国家自然科学基金资助课题(30870205；31070285)；辽宁省教育厅资助课题(L2010516)；辽宁省自然科学基金(20092070,20102205)；沈阳师范大学博士后资助课题 |
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| 中文摘要: |
| 以二叶期水稻幼苗为试验材料,研究其在15%PEG6000模拟的水分胁迫下脯氨酸积累及代谢关键酶活性变化,结果表明:叶片和根系中脯氨酸含量在胁迫后快速积累,胁迫24 h达到最大值,随后逐渐降低;控制脯氨酸合成的关键酶吡咯啉-5-羧酸合成酶(P5CS)和鸟氨酸转氨酶(δ-OAT)活性都呈现先升后降的变化趋势,而控制脯氨酸降解的关键酶脯氨酸脱氢酶(ProDH)活性呈现先降后升的变化趋势。 |
| 英文摘要: |
| Using rice seedlings at two-leaf stage as tested materials, the free proline content and activities of en-zymes involved in proline metabolism were studied under water stress induced by 15%PEG. The results showed that the proline content increased obviously under stress, reached the maximum after 24 h and then decreased. The activities of △1-pyroline-5-carboxylate synthetase (P5CS ) and ornithine- δ-aminotransferase (δ-0AT)， the key enzymes of proline accumulation, increased sigificantly and then declined; while the activity of proline dehydrogenase (ProDH)， a key en-zyme of proline degradation, declined significantly and then increased. |
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