| 任万杰,范锋贵,鲁清林,高正,张晓科,张钰玉.苗期水分胁迫应答蛋白与小麦品种（系）水旱生态型的关系[J].干旱地区农业研究,2011,29(4):1~5 |
| 苗期水分胁迫应答蛋白与小麦品种（系）水旱生态型的关系 |
| Relationship between ecological types of wheat cultivars sown in irrigated and dry fields and their responsive protein at seedling stage under different water stress treatments |
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| DOI：10.7606/j.issn.1000-7601.2011.04.01 |
| 中文关键词: 小麦 水分胁迫 应答蛋白 生态型 |
| 英文关键词:wheat(Triticum aestivum L) water stress responsive protein ecological type |
| 基金项目:国家自然科学基金（30971770）；西北农林科技大学唐仲英育种基金 |
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| 中文摘要: |
| 为了解苗期水分胁迫应答蛋白表达与小麦品种（系）水旱生态型间的关系，以150份不同水旱生态型的小麦品种（系）为材料，采用正常供水和 -1.0 MPa PEG 6000胁迫分别处理其幼苗72 h后，应用SDS-PAGE方法检测叶片内分子量为66.2 kDa左右的应答蛋白在不同品种（系）内的表达，分析该表达蛋白与品种（系）所属生态
型的关系。在水分胁迫与非胁迫两种方式处理幼苗后，该蛋白的表达在供试品种（系）间存在三种类型：25份品种（系）在两种供水条件下均表达该蛋白，占参试品种（系）的16.7%；58份只在水分胁迫条件下表达该蛋白而在非胁迫条件不表达该蛋白，占38.7%；其余67份
品种（系）在两种供水条件下都不表达该蛋白，占44.6%。通过对品种（系）水分胁迫应答蛋白的三种表达类型与其所属水、旱、水旱生态型的相关分析表明，该蛋白表达的类型与品种（系）水旱生态型间存在极显著的相关；这说明苗期水分胁迫应答蛋白的表达与品种（系
）抗旱性密切相关，该蛋白在不同水分条件下表达与否可作为品种（系）抗旱性鉴定的一项指标。 |
| 英文摘要: |
| In order to understand the relationship between expression of responsive protein under different water stress treatments at seedling stage and ecological types of wheat cultivars and advanced lines sown in irrigated, dry or irrigated-dry fields, the seedlings of 150 wheat cultivars and advanced lines with different ecological types were treated with well-watered and -1.0 MPa PEG-6000 treatments for 72 h, respectively. After the SDS-PAGE method was used to detect a 66.2 kDa responsive protein in those cultivars, the relationship between the expression of responsive protein at seeding stage under different water stress treatments and ecological types of those cultivars was analyzed. Cultivars and advanced lines could be divided into three types based on the expression of the responsive protein at seeding stage under different water stress treatments. In all the cultivars and advanced lines，25 of them (16.7%) expressed the responsive protein under both well-watered and -1.0 MPa PEG-6000 treatments, 58 cultivars and advanced lines (38.7%) expressed the responsive protein only under -1.0 MPa PEG 6000 treatment, the other 67 cultivars and advanced lines (44.6%) did not express the protein under the two kinds of water stress treatments. Statistical analysis showed that three expressive types of responsive protein were significantly correlated with three ecological types of cultivars and advanced lines. Those illustrated that the expression of the responsive protein at seedling stage under water stress had a close and positive relationship with the drought resistance of cultivars and advanced lines. Therefore, the expression of the responsive protein could be considered as one of the indexes of drought resistance identification for wheats. |
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