| 陈志远,王国栋,邸丽俊,武永军,杨佳晔.低温冻害对蚕豆幼苗生理生化特性的影响及RAP-PCR指纹分析[J].干旱地区农业研究,2011,29(4):6~12 |
| 低温冻害对蚕豆幼苗生理生化特性的影响及RAP-PCR指纹分析 |
| Effect of low temperature freezing injury on physiological and biochemical characteristics of Vicia faba at the seedling stage and RAP-PCR fingerprint analysis |
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| DOI：10.7606/j.issn.1000-7601.2011.04.02 |
| 中文关键词: 低温胁迫 低温伤害 RAP-PCR 蚕豆 |
| 英文关键词:cold stress freezing injury RAP-PCR Vicia faba |
| 基金项目:国家自然科学基金(30600384) ;西北农林科技大学青年骨干支持项目 |
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| 中文摘要: |
| 0℃冻害分别处理蚕豆幼苗0、3、6、12 h，测定了蚕豆幼苗叶片的蛋白质、脯氨酸、还原性谷胱甘肽和丙二醛等生理指标，进行了蚕豆低温胁迫下的RAP-PCR指纹分析和抵抗低温冻害的特异基因序列片段的克隆。结果表明:随着低温胁迫的加强,蚕豆幼苗叶片内蛋白质含量呈现先升后降的趋势，脯氨酸相对百分含量逐渐增加，还原性谷胱甘肽含量逐渐下降，丙二醛含量低温胁迫后维持在一个较低的稳定水平。以8条随机引物进行RAP-PCR指纹分析鉴定出8条抵抗冻害的特异性片段，并成功克隆到其中的4条。 |
| 英文摘要: |
| Protein content, proline, reduced glutathione, malondialdehyde of Vicia faba at seedling stage were measured in 0℃, RAP-PCR fingerprint analysis was conducted and chilling damage-resistance gene segments were cloned. The results showed that proline increased with the time at 0℃, reduced glutathione decreased with th
e time, protein content increased first but decreased then, and malondialdehyde stayed at a steady level after freezing injury. Eight chilling damage-resistance genes were identified with 8 random primers and RAP-PCR, and four of them were successfully cloned. |
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