| 南炳东,陈耀锋,闵东红,张小红,赵洁岚,薛飞洋.不同水分胁迫下转TaEBP基因小麦株系拔节期抗旱性研究[J].干旱地区农业研究,2011,29(4):23~28 |
| 不同水分胁迫下转TaEBP基因小麦株系拔节期抗旱性研究 |
| Drought resistance of transgenic wheat with TaEBP gene under different water stress during jointing stage |
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| DOI：10.7606/j.issn.1000-7601.2011.04.05 |
| 中文关键词: 水分胁迫 TaEBP 转基因小麦 生理生化指标 抗旱性 |
| 英文关键词:water stress TaEBP transgenic wheat physiological and biochemical indexes drought-resistance |
| 基金项目:转基因生物新品种培育科技重大专项(2009ZX08002-008B)；唐仲英育种基金 |
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| 中文摘要: |
| 以两个转TaEBP基因小麦株系及相应受体对照品种为材料，采用盆栽控水法研究了水分胁迫对转基因小麦拔节期抗旱相关生理生化指标的影响，并结合隶属函数法对转基因株系的抗旱性进行了综合评价。结果显示：随着水分胁迫加剧，各参试品种（系）的相对含水量、叶绿素含量、光合速率、气孔导度呈下降趋势，转基因小麦下降幅度较小；胞间CO2、丙二醛
含量、质膜相对透性呈上升趋势，受体对照品种上升幅度更大；转基因小麦的脯氨酸含量显著高于对照品种。应用隶属函数法综合评价结果表明，转TaEBP基因小麦株系G221、G258比其受体对照具有较高的抗旱能力。 |
| 英文摘要: |
| Using two transgenic wheat strains with TaEBP gene and corresponding recurrent parent as materials, a pot experiment was conducted under different water stress to compare drought-resistance of different varieties by studying the effect of physiological and biochemical indexes on drought resistance of transgenic wheat during jointing stage, and then to make a comprehensive evaluation of drought-resistance with subordination function method. The results showed that with the increase of water stress, leaf relative water content, chlorophyll content, photosynthetic rate and stomatal conductance showed downtrend, and the transgenic wheat declined more slowly; while intercellular CO2, MDA content, plasma membrane permeability showed uptrend, but the corresponding contrast rose more steeply. The proline content of transgenic wheat was significantly higher than contrast varieties. Based on the comprehensive evaluation we found that transgenic wheat strains G221 and G258 with TaEBP gene have higher drought-resistant capability than the corresponding contrast ones XN979 and HG35． |
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