| 代金霞,王玉炯,郭晶静,武雪娟,张晓玲.荒漠植物柠条根瘤菌的抗逆性及其系统发育分析[J].干旱地区农业研究,2011,29(4):223~227 |
| 荒漠植物柠条根瘤菌的抗逆性及其系统发育分析 |
| Analysis of stress resistance and phylogenesis of rhizobia isolated from Caragsana spp. |
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| DOI：10.7606/j.issn.1000-7601.2011.04.41 |
| 中文关键词: 柠条 根瘤菌 抗逆性 系统发育 |
| 英文关键词:Caragsana spp. rhizobia stress resistance phylogenesis |
| 基金项目:宁夏自然科学基金资助项目（NZ1040） |
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| 中文摘要: |
| 对分离自宁夏白芨滩国家级自然保护区的62株柠条根瘤菌的耐盐性、耐酸碱性、生长温度范围和抗生素抗性进行了测定，并采用16S rDNA PCR-RFLP和16S rDNA全序列分析方法对其进行了遗传多样性及系统发育分析。结果表明:柠条根瘤菌具有较强的耐受高温和耐盐、耐酸碱能力，抗逆性较强，但仍存在菌株间差异。70.8%的菌株可耐受4%的NaCl，全部菌株对100 μg/mL的青霉素具有抗性，均可在pH5.0～11.0的培养基中生长。经16S rDNA序列测定鉴定为豌豆根瘤菌的CNXE4、CNXLW24和中华根瘤菌的CNXC3，这3株菌在pH4.0～12.0的范围内都可生长，可以耐受6%的NaCl、50 μg/mL浓度的卡那霉素和链霉素、300 μg/mL的青霉素，在60℃热激后可生长, 表现出极强的抗逆性。16S rDNA PCR-RFLP将62株菌分为7种图谱类型，16S rDNA全序列分析的结果表明, 它们分别归属于根瘤菌属、中华根瘤菌属、土壤杆菌属、叶杆菌属和慢生根瘤菌属，遗传多样性较为丰富。 |
| 英文摘要: |
| Several biochemical characteristics of 62 rhizobia isolated from Caragsana spp. in Baijitan National Nature Reserve in Ninaxia were detected, including resi
stance to salt, acid-alkali, temperature variation and intrinsic antibiotics. The results indicated that 70.8% strains could tolerate NaCl stress at 4% concentration. All of the strains could tolerate Amp stress at 100 μg/mL as well as could grow at a range of pH from 5～11. Strains CNXE4, CNXLW24 and CNXC3 had high resistance, they could grow at pH from 4～12，could tolerate 6% concentration NaCl, 50 μg/mL KM and SM, 300 μg/mL AMP, and could grow after being exposed to 60℃ for 10 min. The methods of 16S rDNA PCR-RFLP and 16S rDNA sequencing were used to analysis the diversity and phylogenesis of 62 rhizobia strains. The results revealed that all the tested strains belonged to the genus of Rhizobium, Sinorhizobium, Agrobacterium, Phyllobacterium and Bradyrhizobium. The rhizobia isolated from Caragsana spp. showed a rich genetic diversity. |
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