蒋花,王占红,张小燕.PEG渗透胁迫下3份大麦材料幼苗叶片抗旱生理特性分析[J].干旱地区农业研究,2011,29(5):100~105
PEG渗透胁迫下3份大麦材料幼苗叶片抗旱生理特性分析
Analysis on drought resistance of leaves of barley seedling under the condition of PEG osmotic stress
  
DOI：10.7606/j.issn.1000-7601.2011.05.18
中文关键词:  渗透胁迫  生理特性  大麦
英文关键词:osmotic stress  physiological characteristics  barley
基金项目:陕西省自然科学基金(2006C120)；唐仲英育种专项
作者单位
蒋花 西北农林科技大学农学院， 陕西 杨凌 712100 
王占红 西北农林科技大学生命学院， 陕西 杨凌 712100 
张小燕 西北农林科技大学农学院， 陕西 杨凌 712100 
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中文摘要:
      以甘啤4号、哈瑞特和D-22大麦为材料，测定不同浓度PEG6000（5%、10%、15%、20%、25%和30%）渗透胁迫处理下大麦幼苗叶片的相对含水量、Pro含量等生理指标，分析PEG渗透胁迫模拟干旱对大麦生理生化特性的影响。结果表明，随着PEG溶液浓度的增加，三个材料的大麦幼苗叶片的相对含水量均下降，而脯氨酸含量、相对电导率、MDA含量、POD活性等均呈上升趋势。低浓度处理下，三个材料的幼苗叶片所测定的生理指标变化幅度小，随着处理浓度的升高变化幅度加大，但不同材料间差异较大。在25%和30%PEG处理下，D-22叶片含水量分别比对照降低53.2%和58.5%，脯氨酸含量比对照高91.9%和92.4%，相对电导率较对照高62.8%和73.5%，MDA分别比对照增加54.1%和61.2%，而甘啤4号叶片含水量仅下降24.3%和42.4%，脯氨酸含量比对照高34.2%和58.0%，相对电导率仅在30%PEG浓度下表现出比对照高72.3%，哈瑞特介于二者之间。过氧化物酶活性表现出甘啤4号最高和D-22最低。结论：三个材料的抗旱性呈现出：甘啤4号＞哈瑞特＞D-22。
英文摘要:
      The relative water content, Pro content, POD and other physiological ndicators in the leaves of barley seedling were measured with the varieties of Ganpi No.4, Harrington and D-22 as materials under the condition of drought stress set with PEG6000. The results showed that for all materials ，by the increasing of PEG 6000 concentration, the relative water content decreased but the relative conductivity, MDA, prolines and POD, four other ndicators showed an increasing trend. All the indicators tested changed small under the teatment of low PEG　concentration．But their changes became big as the treatment concentration increased. However,, there were large differences between different the materials. At the treatment of 25% and 30% PEG concentration, compared with no PEG treatment, the water content of D-22 blades reduced 53.2% and 58.5% respectively, proline content increased 91.9% and 92.4%, relative conductivity enhanced 62.8% and 73.5%, MDA increased 54.1% and 61.2%, respectively, while the water content of Ganpi No.4 blades reduced only 24.3% and 42.4% respectively, proline content increased 34.2% and 58.0%, relative conductivity enhanced 72.3% only at the treatment of 30% PEG, the performance of Harrington was between them. For the POD activity, Ganpi No.4 was highest with D-22 lowest. Conclusion：the ability of Drought resistance for the materials is Ganpi No.4 beer 4＞Harrington＞D-22.
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