| 涂玉琴,戴兴临,涂伟凤,汤洁.芽期PEG模拟干旱胁迫下不同基因型甘蓝型油菜的反应差异研究[J].干旱地区农业研究,2011,29(6):213~221 |
| 芽期PEG模拟干旱胁迫下不同基因型甘蓝型油菜的反应差异研究 |
| Studies on genetic difference of different genotype Brassica napus during germination period under drought stress simulated by PEG |
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| DOI：10.7606/j.issn.1000-7601.2011.06.39 |
| 中文关键词: 甘蓝型油菜 聚乙二醇(PEG) 干旱胁迫 反应差异 种质创新 |
| 英文关键词:Brassica napus polyethylene glycol (PEG) drought stress genetic difference gemplasm creation |
| 基金项目:国家自然科学基金(31060195)；江西省自然科学基金(2009GZN0060,2010GQN0104)；江西省农科院博士启动项目；江西省农科院科技创新基金 |
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| 中文摘要: |
| 采用10%聚乙二醇(PEG-6000)模拟干旱胁迫的方法,对41份来源于甘蓝型油菜与菜属间杂交获得的稳定高世代品系和32份来自不同地区的甘蓝型油菜品种进行芽期模拟干旱胁迫处理,以研究干旱胁迫下不同基因型甘蓝型油菜的反应差异。实验结果表明,PEG模拟干旱胁迫后,大部分材料成苗率降低,平均相对成苗率为96.11%,苗高、苗鲜重和活力指数普遍降低,相对苗高、相对苗鲜重和相对活力指数分别为85.82%、75.65%和82.60%,而根长则明显伸长,相对根长为179.69%;73份供试材料的相对成苗率、相对根长、相对苗高、相对苗鲜重和相对活力指数的变异系数依次为4.33、20.10、16.72、18.73和18.05,不同基因型甘蓝型油菜芽期抗旱性存在较大差异;聚类分析结果,来源于远缘杂交的16份创新种质和8份育成品种为高抗旱类型,可作为油菜抗旱性遗传改良的种质资源加以利用;41份创新种质与32份育成品种芽期抗旱性比较分析表明,创新种质芽期抗旱力高于育成品种,且抗旱遗传差异也较育成品种明显。 |
| 英文摘要: |
| With simulated drought stress in 10%(w/v) polyethylene glycol 6000 (PEG-6000), 41 accessions of high generation lines from intergeneric hybrids between B. napus and Rorippa indica and 32 Brassica napus released cultivars from different areas were treated during gemination period to inv estigate genetic difference of various genotypes of B. napus under drought stress simulated by PEG. The results showed that the survival ratio of most materials reduced
with an average of 96.11%, the seedling height, fresh weight per plant and vigor index also generally reduced, with the relative seedling height of 85.82%，relative fresh weight per plant of 75.65% and relative vigor index of 82.60%. But the taproot length increased significantly， the average relative taproot length reached 179.69%. The variation coefficient of relative survival ratio, relative taproot length， relative seedling height，relative fresh weight per plant and relative vigor index from 73 accessions of germplasm were 4.33, 20.10， 16.72, 18.73 and 18. 05，respectively. The drought toler-ance of different genotypes of B. napus had greater difference during gemination period. And the clustering analysis showed that 16 accessions of new gemmplasm from wide cross and 8 released varieties belonged to high tolerant drought re-sistance types，which could be used as drought tolerant gemplasm for genetic inprovement of B. napus. The comparison between the drought tolerance of 41 accessions of new gemplasm and that of 32 released cultivars during gemmination pe-riod showed that new germplasm from wide cross had better drought tolerance than released cultivars. |
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