郭伟,王庆祥.腐植酸浸种对盐碱胁迫下小麦根系抗氧化系统的影响[J].干旱地区农业研究,2011,29(6):222~226
腐植酸浸种对盐碱胁迫下小麦根系抗氧化系统的影响
Effects of humic acid on activity of antioxidation system in wheat root under salinity alkalinity stress
  
DOI:10.7606/j.issn.1000-7601.2011.06.40
中文关键词:  小麦  根系  盐碱胁迫  抗氧化系统  腐植酸
英文关键词:wheat  root  salinity-alkalinity stress  antioxidation system  humic acid
基金项目:黑龙江省重大课题(G00QY08);黑龙江省教育厅课题(1153lz17)
作者单位
郭伟 沈阳农业大学农学院,辽宁 沈阳 110161
黑龙江八一农垦大学农学院,黑龙江 大庆 163319 
王庆祥 沈阳农业大学农学院,辽宁 沈阳 110161 
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中文摘要:
      采用中性盐(NaCl)和碱性盐(NaHCO3)混合模拟不同浓度盐碱胁迫条件,对龙麦26和克旱16两种不同基因型春小麦进行腐植酸浸种处理。分别测定根系氧离子自由基、丙二醛和谷胱甘肽含量,以及过氧化物酶和超氧化物歧化酶活性,以探讨腐植酸对盐碱两种胁迫下小麦根系抗氧化系统的调控效应。结果表明:随着盐碱胁迫浓度的提高,小麦幼苗根系内丙二醛含量逐渐增加,根系过氧化物酶活性增强,超氧化物歧化酶活性降低,且盐胁迫比碱胁迫影响更大。腐植酸浸种降低了根系活性氧的积累,增强了过氧化物酶和超氧化物歧化酶活性,并促进了谷胱甘肽合成。
英文摘要:
      Neutral salt (NaCl) and basic salt (NaHCO3) were mixed to simulate different concentration salinity al-kalinity stress respectively,two kinds of genotype spring wheat (Longmai 26 and Kehan 16) were treated for soaking seed of humic acid. The content of oxygen free radicals, malondialdehyde (MDA) and glutathione (GSH),and the activity of peroxidase (POD) and superoxide dismutase (SOD) of root were determined to investigate the control effects of HA on an tioxidant system of wheat root under salinity alkalinity stress. The results showed that the content of MDA and the activity of POD were increased, however, the activity of SOD were decreased, and the effect of salt stress was more than that of alkali stress. Soaking seed of humic acid reduced the acumulation reactive oxygen species (ROS),increased the activity of POD and SOD in root,and promoted the synthesis of GSH.
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