黑麦草幼苗对NaHCO<sub>3</sub>胁迫的生理响应

刘建新; 王鑫; 王金成; 李东波; 王凤琴

刘建新,王鑫,王金成,李东波,王凤琴.黑麦草幼苗对NaHCO₃胁迫的生理响应[J].干旱地区农业研究,2012,30(1):138~142

黑麦草幼苗对NaHCO₃胁迫的生理响应

Physiological response of ryegrass seedlings to NaHCO₃ stress

DOI：10.7606/j.issn.1000-7601.2012.01.24

中文关键词: NaHCO₃胁迫黑麦草活性氧代谢生理响应

英文关键词:NaHCO₃ stress ryegrass ROS metabolism physiological responses

基金项目:甘肃省庆阳市科技攻关计划项目(0802NKCM082)

作者	单位
刘建新	陇东学院生命科学与技术学院甘肃省高校陇东生物资源保护与利用省级重点实验室, 甘肃庆阳 745000
王鑫	陇东学院生命科学与技术学院甘肃省高校陇东生物资源保护与利用省级重点实验室, 甘肃庆阳 745000
王金成	陇东学院生命科学与技术学院甘肃省高校陇东生物资源保护与利用省级重点实验室, 甘肃庆阳 745000
李东波	陇东学院生命科学与技术学院甘肃省高校陇东生物资源保护与利用省级重点实验室, 甘肃庆阳 745000
王凤琴	陇东学院生命科学与技术学院甘肃省高校陇东生物资源保护与利用省级重点实验室, 甘肃庆阳 745000

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中文摘要:

采用营养液砂培方法，研究了不同浓度NaHCO₃（0、50、100、150　mmol/L和200 mmol/L）胁迫对黑麦草幼苗生长、活性氧代谢和渗透溶质积累的影响。结果表明：（1）随着NaHCO₃浓度增加，黑麦草幼苗鲜、干重呈降低趋势，叶片超氧阴离子(O₂^·)、过氧化氢(H₂O₂)、丙二醛(MDA) 和Na⁺含量不断升高，过氧化氢酶(CAT)、过氧化物酶(POD)活性和抗坏血酸(AsA)、谷胱甘肽(GSH)、K⁺含量及K⁺/Na⁺持续下降，超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性呈先升后降变化，液泡膜H⁺-ATPase和H⁺-PPase活性在NaHCO₃浓度为50～150 mmol／L时高于对照，NaHCO₃浓度为200 mmol／L 时低于对照。（2）碱胁迫降低了幼苗叶片中可溶性蛋白质和游离氨基酸的含量，提高了可溶性糖和脯氨酸的含量。表明活性氧代谢和Na⁺、K⁺平衡失调及积累有机溶质进行渗透调节需更多能耗等可能是碱胁迫下黑麦草幼苗生长受抑的重要因素。

英文摘要:

Ryegrass seedlings were exposed to Hoagland′s nutrient solution with NaHCO₃ added (0, 50, 100, 150, 200 mmol／L), and the eff ects of NaHCO₃ stress on the plant growth, metabolism of reactive oxygen species (ROS) and osmotica accumulation in leaves were studied by a sandy culture. The results showed (1) With the increasing of NaHCO₃ concentration, the fresh and dry weights of the seedlings decreased, the contents of O₂^·, H₂O₂, MDA and Na⁺ in leaves increased gradually, and the activities of CAT and POD, the contents of AsA, GSH and K⁺ as well as K⁺/Na⁺ decreased continually, and the activities of SOD and APX exhibited a humped-curve, while the activities of V-H⁺-ATPase and V-H⁺-PPase were higher than the control at 50～150 mmol／L　NaHCO₃ but lower than the control at 200 mmol／L　NaHCO₃. (2) Alkali-stress decreased the contents of soluble protein and free amino acid while increased the contents of soluble sugar and proline in the seedling leaves. It was concluded that the imbalances of ROS metabolism and Na⁺ to K⁺ in the seedling leaves were possibly caused by alkaline stress, and consumed more energy for osmotic adjustment by accumulating organic solutes, which might be an important reason that the growth of ryegrass seedlings under alkaline stress was inhibited.

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