| 刘江,谷洁,高华,秦清军,陈胜男,李海龙,张卫娟,郭星亮,张洪宾,陈琳,邵丽.秦岭山区无机磷细菌筛选及其Biolog和分子生物学鉴定[J].干旱地区农业研究,2012,30(1):184~189 |
| 秦岭山区无机磷细菌筛选及其Biolog和分子生物学鉴定 |
| Isolation and identification of inorganic phosphorus-solubilizing bacteria by Biolog and molecular method |
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| DOI：10.7606/j.issn.1000-7601.2012.01.32 |
| 中文关键词: 解磷细菌 鉴定 Biolog 16S rDNA |
| 英文关键词:inorganic phosphate solubilizing bacteria identification Biolog 16S rDNA |
| 基金项目:国家自然科学基金（40771109，40871119） |
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| 中文摘要: |
| 为获得具有高解磷活性，可用于微生物肥料研制和生产的无机磷细菌菌株资源，采用平板溶磷圈法，从秦岭山区植物根际土壤样品中筛选获得3
株高效无机磷细菌，命名为NO.9、NO.15、NO.17，根据钼锑抗比色法，培养5 d后，检测菌株发酵液，确定3株细菌的可溶性磷含量分别为98.12、80.37 mg/L和104.91 mg/L，微生物量磷含量分别为62.85、53.61、36.59 mg/L。生理生化特征、Biolog和16S rDNA鉴定结果表明，3株细菌均属于革兰氏阴性非肠道菌，NO.9菌株为Inquilinus ginsengisoli，NO.15菌株为醋酸钙不动杆菌（Acinetobacter calcoaceticus），NO.17菌株为荧光假单胞菌（Pseudomonas fluorescens）。 |
| 英文摘要: |
| A study was conducted to isolate effective inorganic phosphorus-solubilizing bacteria for potential research and production of biofertilizer. Three strains, named as NO.9, NO15 and NO.17, are isolated from soi
l samples collected from Qinling Mountains by their dissolving phosphorus circle on PKO Agar media. After 5 days incubation, through Mo-Sb colorimetry, the soluble phosphorus content in liquid media is 98.12 mg/L, 80.37 mg/L and 104.91 mg/L respectively, and the content of microbial biomass phosphorus is 62.85 mg/L, 53.61 mg/L and 36.59 mg/L. Analysed by physiology and biochemistry experiments, Biolog and 16S rDNA sequencing reflects that all of the three strains are gram-negative intestinal bacteria. and NO.9 strain is identified as Inquilinus ginsengisoli, NO.15 strain as Acinetobacter calcoaceticus and NO.17 strain as Pseudomonas fluorescens. |
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