| 李小玲,刘雅莉,华智锐.农杆菌介导的ACO基因RNAi载体转化百合抗衰老研究[J].干旱地区农业研究,2012,30(2):88~92 |
| 农杆菌介导的ACO基因RNAi载体转化百合抗衰老研究 |
| Agrobacterium-mediated transformation of RNAi vector with ACO gene on anti-aging of lily |
| |
| DOI：10.7606/j.issn.1000-7601.2012.02.16 |
| 中文关键词: 农杆菌介导 ACO基因 RNAi表达载体 抗衰老 百合 |
| 英文关键词:Agrobacterium-mediated ACO gene RNAi expression vector anti-aging lily |
| 基金项目:国家自然科学基金项目（30371013）；陕西省科技厅项目（2009K01-11） |
|
| 摘要点击次数: 443 |
| 全文下载次数: 334 |
| 中文摘要: |
| 本研究旨在通过根癌农杆菌介导法将ACO基因转入亚洲百合植株中，为植物抗衰老提供理论依据和技术基础。以亚洲百合‘精粹’无菌苗离体小鳞片为外植体，利用农杆菌介导法
将携带有抗衰老基因ACO的RNAi载体转化到百合中，观测分析影响百合遗传转化的因素。结果表明：在遗传转化过程中，预培养3 d后，在菌液和共培养基中均添加25 mg/L乙酰
丁香酮，将OD600=0.8的根癌农杆菌侵染小鳞片8 min,再共培养3 d，有利于获得最高遗传转化效率，抗性植株经PCR检测部分呈现阳性，初步证明ACO基因已经导入到百合基因组中。 |
| 英文摘要: |
| With the scale of asiatic lily as explants, the RNAi vector with ACO anti-aging gene was transferred into lily with Agrobacterium mediated transformation to study the factors which affected the transformation and to provide theoretical an
d technical basis for anti aging of plants. The results showed that the highest transformation efficiency was obtained through the following transformation procedure: after preculture for 3 d, 25 mg/L acetosyringone (AS) was added to Agrobacterium and co-cultivation medium respectively, the explants were inoculated for 8min with diluted OD600=0.8 recombinant Agrobacterium tumefacien culture liquid, and then co-cultivated for 3 d. PCR detection showed that some resistant plants were positive, which indicated preliminarily that the ACO gene was integrated into the genome of transgenic plants. |
| 查看全文 查看/发表评论 下载PDF阅读器 |