| 范惠玲,白生文,张芬琴,肖占文,陈修斌,孙万仓.芸芥自交亲和性的遗传及自交亲和基因mRNA差异显示分析[J].干旱地区农业研究,2012,30(6):23~31 |
| 芸芥自交亲和性的遗传及自交亲和基因mRNA差异显示分析 |
| Analysis of self-compatibility genes by mRNA differential display technique and inheritance of self-compatibility in E.sativa Mill |
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| DOI：10.7606/j.issn.1000-7601.2012.06.04 |
| 中文关键词: 芸芥 自交亲和性的遗传 自交亲和基因 mRNA差异显示技术 |
| 英文关键词:E. sativa Mill inheritanee of self-compatibility self-compatible genes mRNA differential display |
| 基金项目:国家863计划资助项目(2009AA101105)； 河西学院青年教师科研基金项目(QN2011-17) |
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| 中文摘要: |
| 以8份芸芥自交亲和系与4份芸芥自交不亲和系为试验材料,通过正交和反交方式共组配了18个不同的杂交组合,调查了F1群体的自交亲和性与否,及1 424株F2群体和626株BC1群体的性状分离情况。同时,还采用mRNA差异显示技术研究了芸芥自交亲和基因的表达器官,分离、筛选了与自交亲和性相关的特异基因片段。结果表明:芸芥自交亲和性与细胞质遗传无关,而是由一对核基因控制的简单隐性遗传性状;芸芥自交亲和基因的表达不属于组成性表达,而属于组织特异性表达;在芸芥自交亲和系的柱头cDNA扩增中共得到3条差异cDNA片段,其与芸芥自交亲和基因有密切关系。 |
| 英文摘要: |
| 18 hybridized combinations were made between 8 self-compatible lines and 4 self-incompatible lines, and inheritance of self-compatibility in E.sativa Mill was analysed. Furthermore, expresstion tissue of self-compatible genes was preliminary analysed, and specific cDNA fragments related to self-compatibility were isolated and screened by using mRNA differential display technique. The results showed that all the F1 plants were self-incompatible, and the F2 and BC1 population segregated in ratio. Through chi square test, the segregation ratios of self-incompatibility to self-compati-bility in F2 and BC1 population were coincided with the ratio of 1:3 and 1:1, respectively. Therefore, it was inferred that the hereditary of self-compatibility in Yunjie was controlled by one pair recessive nucleus gene. Self-compatible genes of Yunjie was not constitutive expression, but that belonged to specific tissue expression. Self-compatible lines had 3 differ-entially expressed bands which exhibited stably with different primers, these cDNA bands were closely related to self-eompatibility of Yunjie. |
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