| 乔枫,耿贵工,陈志.混合盐碱处理下蚕豆叶片生理指标的变化[J].干旱地区农业研究,2013,31(3):162~165 |
| 混合盐碱处理下蚕豆叶片生理指标的变化 |
| Physiological responses of Vicia faba L.leaves to salt-alkali stress |
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| DOI：10.7606/j.issn.1000-7601.2013.03.26 |
| 中文关键词: 蚕豆(Vieia faba L.) 叶片 盐碱胁迫 生理指标 |
| 英文关键词:Vicia faba L. leaf salt-alkali stress physiological responses |
| 基金项目:国家科技支撑计划“祁连山湿地生态系统修复保护技术集成与示范”(2012BAC08B04) |
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| 中文摘要: |
| 为了研究蚕豆在混合盐碱胁迫下的生理变化,试验采用3种盐碱胁迫方式KCl/NaCl、KCl/Na2CO3、KCl/Na2SO4处理2周,测定蚕豆鲜重、丙二醛和脯氨酸含量、抗氧化酶活性等生理指标。结果表明,随着盐碱胁迫时间的延长,蚕豆鲜重表现为先升高后下降趋势,丙二醛和脯氨酸含量呈上升趋势,其中脯氨酸含量在处理11d时急剧增加；随胁迫时间的延长,4种抗氧化酶SOD、POD、CAT、APX活性增加幅度呈先升高后降低趋势,其中处理11d时,酶活性增加幅度顺序是SOD＞ CAT＞ APX或POD。结论:蚕豆盐碱胁迫中,SOD酶活性发挥首要作用,脯氨酸是一种重要的渗透物质。 |
| 英文摘要: |
| In attempt to study physiologic responses of Vicia faba L.to salt-alkali stress, seedlings of Vicia faba L. were treated with three different eombinations of salts, KCl/NaCl, KCl/Na2CO3,KCI/Na2SO4, for 2 weeks. The fresh weight of seedlings and malondialdehyde(MDA) were then measured, and free proline contents and antioxidant enzymes activity of leaf were further analyzed. The results indicated that with extensions of stress time, malondialdehyde(MDA) and free proline contents were increased, while the freshweight of seedlings was increased at first and then decreased. Free proline contents increased sharply at 11 days of stress. Activities of four antioxidant enzymes, superoxide disrutase(SOD), peroxidase(POD), catalase(CAT), ascorbate peroxidase(APX), were increased at first, were then decreased. At 11 days of stress, order of the activity increments of these four antioxidant enzymes was SOD>CAT>APX or POD. In conclusion, SOD played critical role in the
response of Vicia faba L. to salt-alkali stress, and free
proline an important osmotic substance. |
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