| 陈漫，鲁黎明，王龙昌，郭臻，李宁.转ATCIPK23基因烟草植株钾素营养效率研究[J].干旱地区农业研究,2013,31(4):156~160 |
| 转ATCIPK23基因烟草植株钾素营养效率研究 |
| Research on potassium use efficiency of transgenic tobacco plants carrying ATCIPK23 gene |
| |
| DOI：10.7606/j.issn.1000-7601.2013.04.029 |
| 中文关键词: ATCIPK23 烟草 转基因 钾营养效率 |
| 英文关键词:ATCIPK23 tobacco transgenic potassium absorption efficiency |
| 基金项目:国家自然科学基金项目（31271673）；国家自然科学基金项目（30871474）；重庆市科技攻关项目（CSTC，2008AB1001） |
|
| 摘要点击次数: 1280 |
| 全文下载次数: 1221 |
| 中文摘要: |
| 通过考察转ATCIPK23基因烟草植株在不同钾浓度下钾及干物质积累的能力，研究了外源基因的导入对转基因烟草植株钾吸收能力的影响。结果表明，在低钾环境下，转基因株系具有明显的生长优势，烟株发育较快、根系发达、生物量较大、植株的含钾率也有明显提高，随着供钾量的减少，这种差异幅度逐渐增大。当供钾浓度为0.1 mmol·L-1时，对照品种K326植株的平均钾含量为17.7 g·kg-1，而转基因品系KA12为23.3 g·kg-1，KA16为24.6 g·kg-1，KA21为22.1 g·kg-1，分别较对照品种增加了31.6%、39.0%和27.7%。钾吸收动力学研究表明，转基因植株与对照品种植株的最大吸收效率（Vmax）差别不大，而最低浓度（Cmin）与米氏常数（KM）的差别较为明显。 |
| 英文摘要: |
| The experiment was conducted to study the ability of K+ and dry matter accumulation of ATCIPK23 transgenic tobacco lines under different K+ concentration. The results showed that there were remarkable growth advantages for the transgenic tobacco lines, whose plant growth rate, root development, dry matter accumulation and plant potassium content were wholly impr
oved remarkably, compared with the contrast variety. These effects tended to become stronger along with the reduction of potassium supply. When the K+ concentration of culture medium dropped to 0.1 mmol·L-1, the potassium content in the above-ground part of contrast variety K326 was 17.7 g·kg-1, and that of the transgenic lines KA12, KA16 and KA21 was 23.3 g·kg-1, 24.6 g·kg-1and 22.1 g·kg-1 respectively, being increased by
31.6%, 39.0% and 27.7% respectively. The study on potassium absorption kinetics indicated that there was no significant difference in maximum absorption efficiency (Vmax) between the transgenic lines and the contrast variety, but there were significant differences in minimum concentration (Cmin) and Michaelis constant (KM) between them. |
| 查看全文 查看/发表评论 下载PDF阅读器 |