曹君迈.抗生素浓度对不同品种马铃薯脱毒苗农艺性状的影响[J].干旱地区农业研究,2013,31(5):99~105
抗生素浓度对不同品种马铃薯脱毒苗农艺性状的影响
Effect of antibiotic concentration on agronomic traits of different varieties virus free potato plantlets
  
DOI:10.7606/j.issn.1000-7601.2013.05.017
中文关键词:  马铃薯  品种  抗生素  生长
英文关键词:potato  variety  antibiotic concentration  growth
基金项目:国家民委生态模型与应用重点实验项目“马铃薯优质 种苗调控与原原种结薯动态的研究”(2012SY04)
作者单位
曹君迈 (北方民族大学生物科学与工程学院, 宁夏 银川 750021) 
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中文摘要:
      以马铃薯不同基因型品种脱毒基础苗为试验材料,采用两因素随机区组设计,研究不同基因型品种、不同抗生素浓度对马铃薯脱毒基础苗苗鲜重、根鲜重、苗干重、根干重、叶片数、节数、株高、茎粗、根长和根条数等农艺性状影响,培养14 d时,测定各性状指标。结果表明:3个不同成熟期基因型品种对测定的10个农艺性状指标影响显著(P<0.05);4种抗生素浓度(0、20、40、60 mg·L-1)除了茎粗 ,对其它9个农艺性状指标影响显著(P<0.05),适宜的抗生素浓度范围为0 mg·L-1和20 mg·L-1;3个不同成熟期基因型品种、4种不同抗生素浓度处理对马铃薯 单株苗鲜重、根鲜重、苗干重、根干重、节数、株高、茎粗、根条数影响有显著性交互作用 (P<0.05),叶片数、根长不受基因型品种和抗生素浓度交互作用影响;早熟基因型品种 大西洋、中熟基因型品种克新1和晚熟基因型品种青薯168适宜的抗生素浓度为0、20 mg·L -1,各农艺性状表现好。为预防污染发生,降低生产成本,建议继代3-5代时,加入抗 生素20 mg·L-1
英文摘要:
      The different varieties of virus free potato plan tlets were selected as the meterials, and using the method of randomized block d esign of two factor to study the effects of genotypes and different antibiotic concentrations on the fresh weight of shoot and root, the dry weight of shoot an d root, leaf number, node number, plant height, stem diameter, root length and r oot number of individuals after 14 d culture. Our results showed that genotypes had significant effects on the ten agronomic traits (P<0.05). Four kinds of antibiotic concentrations(0、20、40、60 mg·L-1)had notable interacti on effects on other agronomic characters eccept stem diameter (P<0.05), the suitable antibiotic concentrations were 0 mg·L-1 and 20 mg·L-1. Ex cept for leaf number and root length properties index, the genotypes and antibio tic concentrationshad notable interactions on other agronomic characters eccept (P<0.05). The suitable antibiotic concentrations for the early maturing va rietie “Atlantic”, medium maturing varietie “Kexin 1” and late maturing va rietie “Qingshu 168” were 0 mg·L-1 and 20 mg·L-1, to prevent the pollution and production reduction, we suggested to add 20 mg·L-1 antibi otic if subcultured for 3-5 generations.
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