范惠玲，王文倩，李彦龙，白生文.芸芥自交不亲和等位基因及其表达初步研究[J].干旱地区农业研究,2013,31(5):110~116
芸芥自交不亲和等位基因及其表达初步研究
Study on S alleles and their expression in self incompatible lines of Eruca Sativa Mill.
  
DOI：10.7606/j.issn.1000-7601.2013.05.019
中文关键词:  芸芥  自交不亲和系  S等位基因  基因表达  DDRT-PCR
英文关键词:Eruca sativa Mill.  self incompatible lines  S al leles  gene expression  DDRT-PCR
基金项目:河西学院大学生科技创新项目（2011-101）；河西学 院青年教师科研基金项目（QN2011-17）
作者单位
范惠玲，王文倩，李彦龙，白生文 （河西学院农业与生物技术学院， 甘肃 张掖 734000） 
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中文摘要:
      在利用芸芥自交不亲和系途径开展育种研究的过程中 ，为了避免在人工试配组合时，双亲蕾期杂交结籽率较好，但在自然隔离条件下配制杂交种 时出现双亲花期交配不亲和的现象，本试验对10份芸芥进行了系内株间和系间的完全双列杂 交，同时对自交不亲和基因的表达器官进行了DDRT-PCR扩增研究。结果表明，芸芥1、芸芥3 、芸芥5、芸芥6、芸芥8、芸芥9和芸芥13这7份材料系内株间异交和套袋自交的亲和指数均 小于1，即表现为不亲和性，说明这些材料属于S等位基因纯合的不亲和系；而芸芥2、芸芥4 和芸芥10这三份材料系内株间异交和套袋自交时，呈现出不亲和性不稳定的现象，即一部分 杂交表现为亲和，另一部分杂交表现为不亲和，说明这3份材料的自交不亲和性尚未稳定。D DRT-PCR扩增结果表明，芸芥自交不亲和S基因只在柱头组织中表达，其属于组织特异性表达 。因此，在利用自交不亲和系途径培育一代杂种时，在农艺学性状和自交不亲和性基本稳定 时，有必要进一步测定自交不亲和系S等位基因的纯合性和基因型，这一工作可以有效地指 导育种实践。
英文摘要:
      In the process of breeding research by ways of sel f incompatible lines in E. Sativa Mill., there is often a phenomenon that t he seed setting percentage from the cross of two parents is high in bud stage, b ut the number of seeds is low in late stage owing to the natural segregation. By using the method of complete diallel crossing, the purity of S alleles in 10 se lf incompatible lines of E. Sativa Mill. was studied, and the expression ti ssue of self incompatible genes was analyzed with DDRT-PCR technique. The resul ts showed that the self compatible index of Yunjie 1, Yunjie 3, Yunjie 5, Yunji e 6, Yunjie 8, Yunjie 9 and Yunjie 13 was below 1 after selfing or outcrossing among these 7 self incompatible lines, that is to say, their self incompatibil ity was stable. However, the self incompatibility of Yunjie 2, Yunjie 4 and Yun jie 10 was not stable. DDRT-PCR analysis showed that the self incompatible gene s of E. Sativa Mill. didn’t belong to constitutive expression, but to speci fic tissue expression. Therefore, when creating F1 hybrids by ways of self inco mpatible lines, it is necessary for us to determinate the purity of S alleles an d their genotypes besides agronomic traits and self incompatible stability.
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