| 何 军，浦 俊，王渭玲，高青鸽.引发对黄芪种子萌发及幼苗抗旱性的影响[J].干旱地区农业研究,2013,31(5):233~237 |
| 引发对黄芪种子萌发及幼苗抗旱性的影响 |
| Effects of different priming treatments on germination and seedling drought resistances of Astragalus membranaceus (Fisch) Bge. |
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| DOI：10.7606/j.issn.1000-7601.2013.05.039 |
| 中文关键词: 黄芪 引发处理 萌发 抗旱性 |
| 英文关键词:Astragalus membranaceus (Fisch.)Bge.seed priming treatments permination drought resistance |
| 基金项目:“十二五”国家科技支撑计划（2011BAD29B04）；陕西省科技统筹创新工程（2011KTCL02-02） |
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| 中文摘要: |
| 本研究目的在于分析种子引发处理后黄芪发芽特性及幼苗抗旱性，探索提高黄芪种子萌发率的方法，为人工栽培黄芪提供技术支撑。采用20% PEG-6000、0.25% KNO3、100 mg·L-1 GA3、12.5 mg·L-16-BA及H2O（对照）5种化学物质引发处理黄芪种子，进行萌发试验及干旱胁迫，测定种子萌发特性及有关抗旱生理指标。结果表明：各处理发芽势、发芽率的一致顺序为12.5 mg·L-16-BA
＞100 mg·L-1 GA3＞0.25% KNO3＞20% PEG-6000＞H2O。幼苗丙二醛(MDA)含量、可溶性蛋白含量、脯氨酸含量、叶绿素含量、超氧化物歧化酶活性（SOD）、过氧化物酶活性（POD）和过氧化氢酶活性（CAT）与CK之间存在显著差异。比较各处理黄芪幼苗的隶属函数平均值可看出，不同引发处理黄芪种子，幼苗的抗旱性顺序为H2O＞20% PEG-6000＞0.25% KNO3≈12.5 mg·L-1 6-BA＞CK＞100 mg·L-1 GA3。结论：采用20% PEG-6000或0.25% KNO3作为引发剂处理黄芪种子，可有效促进黄芪种子萌发和提高幼苗抗旱性。 |
| 英文摘要: |
| Objective: Physiological effects of seed priming on drought resistance of Astragalus membranaceus (Fisch.) Bge. seedlings were studied in this paper. Method: After Astragalus membranaceus (Fisch.) Bge.seeds were subject to different priming with H2O, 20% PEG-6000,0.25% KNO3,100 mg·L-1 GA3 and 12.5 mg·L-1 6-BA respectively for germination experiment, seedlings were under 20% PEG-6000 simulated drought stress to measure d
rought related physiological indicators. Results: The sequence of Germination energy and germination percentage was identical: 12.5 mg·L-1 6-BA＞100 mg·L-1 GA3＞0.25% KNO3＞20% PEG-6000＞H2O＞CK; and the sequence of Simplified vigor index was 0.25% KNO3＞100 mg·L-1 GA3≈20% PEG-6000＞H2O＞12.5 mg·L-1 6-BA＞CK. Malondialdehyde (MDA) content, soluble protein content, proline content, chlorophyll content, superoxide dismutase (SOD), peroxidase(POD) and catalase activity (CAT) of seedling have a significant difference between CK.According to comparing the Average value of subordinate function, the rank of drought resistance was H2O＞20% PEG-6000＞0.25% KNO3≈12.5 mg·L-1 6-BA＞CK＞100 mg·L-1 GA3. Conclusion: 20% PEG-6000, or 0.25% KNO2 can effectively promote seed germination and improve seedling drought resistance. |
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