宋兵兵，闵东红，徐兆师，陈 明，李连城，马有志.两个转W23基因小麦株系的抗旱性鉴定[J].干旱地区农业研究,2014,32(1):258~262
两个转W23基因小麦株系的抗旱性鉴定
Identification of drought resistance of two transgenic wheat lines with W23 gene
  
DOI：10.7606/j.issn.1000-7601.2014.01.043
中文关键词:  干旱胁迫  W23  转基因小麦  根系  抗旱性
英文关键词:drought stress  W23  transgenic wheat  root  drought resistance
基金项目:国家863计划项目(2011AA100501)
作者单位
宋兵兵1，闵东红1，徐兆师2，陈 明2，李连城2，马有志2 (1.旱区作物逆境生物学国家重点实验室,西北农林科技大学农学院， 陕西 杨凌 712100； 2.中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程,农业部麦类生物学与作物遗传育种重点实验室， 北京 100081) 
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中文摘要:
      以两个T5代转W23基因小麦株系G19-X59、G19-X61为材料，在水培条件下采用PEG-6000人工模拟干旱胁迫措施对转基因小麦株系的根系发育特点、抗旱相关的光合生理等指标进行了测定。结果表明，干旱胁迫条件下各参试小麦的光合速率(Pn)和蒸腾速率(Tr)均下降，转基因株系降幅较小，且其Pn和Tr值极显著高于受体品种；在正常供水和干旱胁迫两种水分处理下，转基因株系均比受体品种具有较发达的根系，其根总长、根总表面积、根总体积等参数极显著高于受体品种。这一结果说明，转W23基因小麦株系G19-X59、G19-X61在苗期依靠发达的根系维持较强的光合作用，提高其应对干旱胁迫的能力。
英文摘要:
      Two T5 transgenic wheat lines G19-X59 and G19-X61 with W23 gene were used as materials, 15% PEG-6000 was used to simulate the drought stress in hydroponic culture, the root development characteristics of transgenic wheat lines and photosynthetic physiology indexes related to drought resistance were investigated. The results indicated that photosynthetic rate (Pn) and transpiration rate (Tr) of all the wheat materials decreased under drought stress, whereas those of the transgenic wheat lines were not significantly changed. The Pn and Tr values of transgenic wheat lines were extremely significantly higher than that of the control Jimai 19. The transgenic lines were also found to have more developed root system than the control under both normal water supply and drought stress treatments. There were also extremely significant difference in total root length, root surface area and root volume between transgenic lines and the control. The results showed that the transgenic lines G19-X59 and G19-X61 could maintain high level of photosynthesis and thereby have strong resistance to drought stress.
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