于艳杰,史振声,胡海军,李 云,王 健.不同抗性糯玉米近等基因系水培幼苗对烟嘧磺隆的反应[J].干旱地区农业研究,2015,33(2):87~90 |
不同抗性糯玉米近等基因系水培幼苗对烟嘧磺隆的反应 |
Responses to nicosulfuron of hydroponic seedlings of near-isogenic lines of waxy corns with different resistances |
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DOI:10.16302/j.cnki.1000-7601.2015.02.014 |
中文关键词: 糯玉米 烟嘧磺隆 叶绿素 SOD POD |
英文关键词:waxy corn nicosulfuron chlorophyll content SOD POD |
基金项目:辽宁省科技计划项目(2011201018);“十二五”国家科技支撑计划项目(2011BAD16B12,2012BAD04B00) |
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中文摘要: |
以抗和不抗烟嘧磺隆的沈糯509R和沈糯509S为试材,通过向水培液添加烟嘧磺隆的方法,研究抗系与不抗系的幼苗外观表现和生理指标变化。结果表明:处理后两个近等基因系均比对照发生明显变化;各项指标随处理浓度的增加而反应加剧;抗系0.02 mL·L-1烟嘧磺隆培养液中甚至无明显反应,而不抗系反应强烈直至死亡;抗系与不抗系对烟嘧磺隆反应的快慢和变化趋势存在明显差异。试验证明,采用0.02 mL·L-1烟嘧磺隆水培液对自交系进行烟嘧磺隆抗性鉴定,无论以形态指标还是以生理指标都可得到准确的鉴定结果。 |
英文摘要: |
To evaluate the morphological and physiological index variations between seedlings exhibiting sensitive and resistant capabilities to nicosulfuron, near-isogenic lines of waxy corns, shennuo 509R and shennuo 509S, were employed for analyses using hydroponic method with the addition of nicosulfuron. The results indicated that these two near-isogenic lines both were significantly different from the control. All responding indexes became intensified with the increase of nicosulfuron concentrations. The resistant line did not have a clear reaction to 0.02 mL·L-1 nicosulfuron, whereas the sensitive line showed a strong response, which became even dead. The sensitive and resistant lines were found to display significant variations in sensitivities and levels to nicosulfuron. In conclusion, the utilization of hydroponic method with 0.02 mL·L-1 nicosulfuron provided an accurate evaluation of inbred lines in responses to nicosulfuron on the basis of their morphological and physiological indexes. |
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