| 杨 刚,王丽萍,孙万仓,曾秀存,刘自刚,李学才,方 彦,武军艳,赵艳宁,董红业,王 月.白菜型冬春性油菜杂交后代抗寒性变异分析[J].干旱地区农业研究,2015,33(2):95~101 |
| 白菜型冬春性油菜杂交后代抗寒性变异分析 |
| Analyses of cold tolerance variations in filial generations of winter and spring turnip rape (Brassica rapa L.) |
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| DOI：10.16302/j.cnki.1000-7601.2015.02.016 |
| 中文关键词: 白菜型油菜 杂交后代 抗寒性 生理生化特性 越冬率 |
| 英文关键词:Brassica rapa L. Filial generations Cold tolerance Physiological indexes Overwintering rates |
| 基金项目:国家“863”高技术研究发展计划（2011AA10A104）；国家“973”重点基础研究发展计划（2015CB150206）；国家公益性行业（农业）科研专项（200903002-04；现代农业产业技术体系建设专项（CARS-13）；国家自然基金 (31460356)；国家农业科技成果转化项目（2014G10000317） |
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| 中文摘要: |
| 选用抗寒性强的白菜型冬油菜（陇油7号和陇油9号）与优质春油菜（Parkland）杂交的后代群体（F1，F2和BC1）为研究对象，以降温前后的油菜SOD、POD、CAT、可溶性蛋白和MDA等5个生理生化特性的变异情况为依据，结合越冬率数据对杂交后代进行抗寒性分析。研究表明：降温后，所有单株的生理生化指标都增加。降温前SOD、POD、CAT、可溶性蛋白和MDA值分别介于131.16～489.50 U·g-1、8.59～68.44 U·g-1、2.63～20.85 U·g-1、2.53～7.80 mg·g-1和3.69～13.67 mg·g-1。降温后，分别为290.87～796.88 U·g-1、22.63～101.5 U·g-1、8.89～48.30 U·g-1、4.46～10.68 mg·g-1和5.77～21.25 mg·g-1。生理生化指标和越冬率均发生分离，F1代生理生化变异系数小，而以冬性亲本为母本的后代越冬率(70.27%)高于春性亲本为母本的后代(64.48%)。F2代和BC1代变异较大，其中BC1代中以冬性亲本为轮回亲本的群体越冬率(71.43%～76.19%)明显高于以春性亲本为轮回亲本群体的越冬率(38.46%～43.75%)。相关性分析表明这5个生理生化特性与越冬率极显著相关,相关系数分别为0.597、0.757、0.665、0.567和-0.663。分析结果表明各项生理生化指标结合越冬率对冬春杂交后代的抗寒性分析是可行的，能够筛选出抗寒优良单株，为白菜型冬油菜品质改良提供基础材料。 |
| 英文摘要: |
| The winter and spring turnip rape parents (Longyou 7, Longyou 9 and Parkland) and their filial generations, significantly differing in cold tolerance and quality, were comprehensively analyzed. Cold tolerance variations were examined on the basis of five physiological indexes and overwintering rates before and after cooling. The results showed that the physiological indexes of all individuals were increased after cooling. The SOD, POD, CAT, soluble protein and MDA values were 131.16～489.50 U·g-1, 8.59～68.44 U·g-1, 2.63～20.85 U·g-1, 2.53～7.80 mg·g-1 and 3.69～13.67 mg·g-1 before cooling, but reached to 290.87～796.88 U·g-1, 22.63～101.5 U·g-1, 8.89～48.30 U·g-1, 4.46～10.68 mg·g-1 and 5.77～21.25 mg·g-1 respectively after the treatment. In addition, physiological indexes and overwintering rates were segregated. The variation of physiological coefficient in F1 generation was small, and the overwintering rates of filial generations of winter turnip rape for female parents (70.27%) were higher than those of the spring parent for the female parent (64.48%). The variations in F2 and BC1 generations were large, and overwintering rates of a group of winter parents for the recurrent parent (71.43%～76.19%) were significantly higher than the groups of spring parents as the recurrent parent (38.46%～43.75%) in BC1. It was further found that significant correlations between the five physiological characteristics and overwintering rates could be identified. Correlation coefficients were 0.597, 0.757, 0.665, 0.567 and -0.663 respectively. In conclusion, it was feasible to analyze cold tolerance characteristics using these physiological indexes, which could reveal the differences of cold tolerance indexes and overwintering rates in different filial generations, and could provide good base materials for quality improvement of winter turnip rape. |
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