| 关小敏,孟品品,刘星,邱慧珍,张文明,海龙,张俊莲,王蒂,沈其荣.马铃薯黑痣病生防菌的筛选和鉴定及其生长条件的研究[J].干旱地区农业研究,2015,33(3):90~95 |
| 马铃薯黑痣病生防菌的筛选和鉴定及其生长条件的研究 |
| Isolation and identification of antagonistic strains against black scurf of potato and conditions for their growth |
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| DOI：10.7606/j.issn.1000-7601.2015.03.15 |
| 中文关键词: 马铃薯 黑痣病 拮抗菌 筛选鉴定 生长条件 |
| 英文关键词:potato black scurf antagonistic bacteria isolation and identification growth condition |
| 基金项目:公益性行业(农业)科研专项(201103004)；国家自然科学基金（31360500）；国家科技支撑计划（2012BAD06B03）；甘肃省科技重大专项(1102NKDM025) |
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| 中文摘要: |
| 从甘肃省景泰县条山农场不同连作年限的马铃薯地块中取根际土样品，分离筛选马铃薯黑痣病病原菌-立枯丝核菌（Rhizoctonia solani）的拮抗菌。采用土壤平板稀释、对峙法筛选拮抗菌；结合形态特征、生理生化及16SrDNA分子鉴定；采用单因素试验设计对G1菌株的生长条件进行初步试验。通过初筛和复筛，从108株细菌中获得1株对立枯丝核菌拮抗效果稳定、拮抗能力较强的细菌G1。采用平板对峙法测定其拮抗作用，抑菌带宽度达6mm，抑菌圈直径＞2.5 cm，抑菌率为66.7%。结合形态特征、生理生化及16SrDNA分子鉴定，G1为类芽孢杆菌（Paenibacillus sp.）；采用单因素试验设计对G1菌株的生长条件进行初步试验。结果表明，菌株G1在4℃～45℃，pH 5～10，盐浓度0～50 g·L-1都能生长，碳源、氮源利用广泛，最适条件为：温度37℃，pH 7，蔗糖为碳源，蛋白胨为氮源，盐浓度为10 g·L-1。拮抗菌G1被鉴定为类芽孢杆菌，该菌株抑菌带较宽、抑菌效果稳定，生长条件与马铃薯生长的大田环境基本相符，具有作为一株优良生防菌的必要条件。 |
| 英文摘要: |
| Bacteria with antagonistic activity against potato black scurf pathogen (Rhizoctonia solani) were isolated from the rhizosphere soils with different years of continuous cropping in hill farm of Jingtai county, Gansu Province. Antagonistic bacteria were isolated from soil through dilution plates and antagonistic plates. Based on the morphological characteristics, physiological and biochemical tests, and 16SrDNA sequencing analysis were performed. Single factor experiment design was carried out to discuss the growth conditions of strain G1. One of the isolated strains, G1, which showed the strongest antagonism, was selected from 108 bacterial strains. It produced an inhibition zone with 6.0mm width and > 2.5cm diameter, and an inhibitory rate at 66.7% through confronting incubation. According to the morphological characteristics, physiological and biochemical tests, and 16SrDNA sequence analyses, it was preliminarily identified to be Paenibacillus sp. In addition, a single factor experiment design was carried out to examine the optimal growth conditions for G1. The results showed that the bacteria could grow at 4℃～45℃ with pH 5～10 and salt concentration 0 to 50 g·L-1. The optimal conditions for G1 growth was found to be 37℃ inoculation with pH at 7 using sucrose and peptone as carbon and nitrogen sources respectively, as well as with a salt concentration at 10 g·L-1. In conclusion, the newly selected Paenibacillus sp. G1 showed broad inhibition zone, with a stable effect against potato black scurf pathogen (Rhizoctonia solani). The growth condition for G1 was similar to those for potato growth, owning necessary criteria as a good biocontrol strain. |
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