| 金喜军,曲春媛,赵云娜,栗文霞,张玉先.锰胁迫对大豆氮素吸收利用的影响[J].干旱地区农业研究,2015,33(4):166~172 |
| 锰胁迫对大豆氮素吸收利用的影响 |
| Effects of manganese stress on assimilation of mineral nitrogen in soybean |
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| DOI：10.7606/j.issn.1000-7601.2015.04.26 |
| 中文关键词: 锰胁迫 大豆 矿质氮 硝酸还原酶 硝态氮含量 |
| 英文关键词:manganese stress soybean mineral nitrogen NR NO3-N content |
| 基金项目:国家自然科学基金项目(204134008)；黑龙江八一农垦大学博士启动基金(XYB2013-03)；黑龙江八一农垦大学作物学科学技术骨干科研启动金项目(ZWXQDJ-4) |
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| 中文摘要: |
| 采用砂培与同位素标记的方法，以最适锰浓度为对照，设置重度锰缺乏（对照浓度的1/25，S1表示）、中度锰缺乏（对照浓度的1/5，S2表示）、中度锰过量（对照浓度的5倍，S3表示）、重度锰过量（对照浓度的25倍，S4表示）4个不同锰胁迫处理，研究了锰胁迫对大豆矿质氮积累的影响。结果表明：不同锰胁迫处理对除苗期外其他时期大豆叶片硝酸还原酶(NR)起显著抑制作用，减小幅度范围15.26％～53.10％，并且抑制作用随胁迫程度的加深而加重；不同锰胁迫处理降低了苗期和成熟期叶、茎、根和成熟期荚果中硝态氮含量，而对其他时期则存在一定促进作用；不同锰胁迫处理降低了苗期至鼓粒期大豆各营养器官和成熟期根中氮素含量，而增加了成熟期叶和茎中氮素含量；锰胁迫处理对结荚期和鼓粒期荚果氮素含量无影响，但显著降低了成熟期荚果氮素含量；不同锰胁迫处理均对苗期和成熟期叶部矿质氮积累无影响，但显著抑制了盛花期至鼓粒期矿质氮积累，S4处理减小幅度最大，为37.34%；不同锰胁迫处理未影响苗期和盛花期茎、根部矿质氮的积累，但显著抑制了结荚期至成熟期的积累；锰胁迫显著抑制了荚果和全株矿质氮的积累，成熟期S1、S2、S3和S4处理荚果和全株矿质氮积累量分别较对照减少了40.93%、22.85%、26.83%和42.15%，以及34.27%、18.38%、22.05%和34.19%。综合分析表明，锰缺乏和锰过量胁迫均明显影响大豆矿质氮的同化和积累，抑制作用随胁迫程度的加深而加重。 |
| 英文摘要: |
| To investigate the effects of manganese on assimilation of nitrogen in soybean, sand culture method with 15N tracer technique was employed in this study, using optimal Mn concentration as a control to evaluate the results from four manganese stress treatments including 2 manganese deficiency treatments (including serious stress and light stress which were 1/25 (S1) and 1/5 (S2) of optimal Mn concentration respectively) and 2 excess stress treatments (including serious stress and light stress which were 5 (S3) and 25 (S4) times of optimal Mn concentration respectively). The results showed that manganese stress had no significant effects on the activities of NR at seedling stage, but showed significant inhibitory effects at the other growth stages (P＜0.01) with decreasing rates at 15.26％～53.10％. The higher the stress level was, the more severe the inhibitory effects were. Different manganese stresses inhibited NO3-N content in leaves, stems and roots at seedling and mature stage and in pods at mature stage, and caused promotion effects at other stages. N contents in vegetative organs during the period from seedling to seed filling stage and in toots at mature stage were inhibited by manganese stress, and were promoted in leaves and root at mature stage. Manganese stress had no significant effects on N contents in pod at pod beginning and seed filling stages, but decreased the N level significantly at mature stage. Manganese stress had no significant effect on mineral nitrogen accumulation in leaves at seedling and mature stages, but inhibited that at full flowering and seed filling stages, with a maximal decreasing rate by S4 at 37.34%. Mineral nitrogen accumulation in stems and roots were not affected by different manganese stress during the period from pod bearing and mature stages. Mineral nitrogen accumulations in pod and the whole plant were also inhibited by manganese stress, displaying decreasing rates by S1, S2, S3 and S4 at mature stages as 40.93%, 22.85%, 26.83% and 42.15%, and 34.27%, 18.38%, 22.05% and 34.19%, respectively. The result indicated that assimilation and accumulation of mineral nitrogen were inhibited by deficient and excessive manganese significantly, and inhibitory effects became increased with the increases of stress levels. |
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