胶质芽孢杆菌Bacillus mucilaginosus的分离及对荒漠沙中棉花种子发芽的影响

吴江利; 罗学刚; 李保强; 李梓番; 杨 圣; 钟雪梅; 吴虹丽

吴江利,罗学刚,李保强,李梓番,杨圣,钟雪梅,吴虹丽.胶质芽孢杆菌Bacillus mucilaginosus的分离及对荒漠沙中棉花种子发芽的影响[J].干旱地区农业研究,2015,33(6):77~82

胶质芽孢杆菌Bacillus mucilaginosus的分离及对荒漠沙中棉花种子发芽的影响

Isolation of Bacillus mucilaginosus and its influence on cotton seed germination in the desert sand

DOI：10.7606/j.issn.1000-7601.2015.06.13

中文关键词: 解钾菌鉴定棉花发芽率超氧化物歧化酶（SOD）荒漠沙

英文关键词:potassium bacteria identification cotton germination rate superoxide dismutase desert sand

基金项目:国家核设施退役及放射性废物治理科研重点项目(14zg6103)；国家农业科技成果转化资金项目(2012GB2G200469)；西南科技大学研究生创新基金资助(14ycx071)

作者	单位
吴江利	西南科技大学生命科学与工程学院，四川绵阳 621010；生物质材料教育部工程研究中心，四川绵阳 621010
罗学刚	生物质材料教育部工程研究中心，四川绵阳 621010
李保强	新疆慧尔农业科技股份有限公司，新疆昌吉 831100
李梓番	西南科技大学生命科学与工程学院，四川绵阳 621010；生物质材料教育部工程研究中心，四川绵阳 621010
杨圣	西南科技大学生命科学与工程学院，四川绵阳 621010；生物质材料教育部工程研究中心，四川绵阳 621010
钟雪梅	西南科技大学生命科学与工程学院，四川绵阳 621010
吴虹丽	成都学院(成都大学)期刊中心，四川成都 610052

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中文摘要:

对采集的土壤样品中解钾菌进行分离与鉴定，再以荒漠沙为土壤基质，加入含菌量为0、0.16、0.48、0.80、1.20、1.60（×10⁸/CFU·mL^-1）的菌液进行发芽试验，研究了解钾菌对棉花种子的发芽率、幼苗的鲜重、芽长和根长、超氧化物歧化酶（SOD）、过氧化物酶（POD）和丙二醛（MDA）含量的影响。结果表明，分离出的解钾菌为胶质芽孢杆菌（Bacillus mucilaginosus）。当沙漠土壤中加入不同含菌量的菌液后：棉花种子发芽时间提前0～24 h，发芽率随着菌含量的增加呈先增大后减少的趋势，当菌含量为0.8×10⁸ CFU·mL^-1时发芽率最高为70.29%；茎的长度增加0.5～1.2 cm，主根的长度缩短0.20～0.65 cm，但促进了侧根的生长，幼苗的鲜重提高了0.0662～0.1582 g；幼苗中的相关酶和丙二醛含量分别有不同程度的增加和减少。

英文摘要:

The potassium bacteria obtained from collected soil samples were isolated and identified, and then the effects of different contents from viable bacteria on the cotton seed germination rate, fresh weight, shoot and root length of seeding, the content of superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA) were studied using desert sands as soil matrixes. The results showed that the potassium bacteria was Bacillus mucilaginosus. When different bacterial contents in bacterial liquid were added into desert soil, germination time of cotton seeds was 0～24 hours earlier than the control experiment. Germination rate showed a tendency to become increased first and then went decreased with the addition of bacterial content, reaching a highest value of 70.29% when the bacterial content was 0.8×10⁸ CFU·mL^-1. The length of the cotton stem and taproot were relatively increased by 0.5～1.2 cm and shortened by 0.20～0.65 cm, respectively. However, the growth of lateral root was promoted, and the fresh weight of seedlings was increased by about 0.0662～0.1582 g. In the meantime, both the content of related enzymes and malondialdehyde in the seedlings became increased or decreased to some extent.

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